磁性纳米粒子的制备及环糊精葡萄糖基转移酶的固定化  

Preparation of aminosilanized magnetic nanoparticles and immobilization of cyclodextrin glucosyltransferase

作　　者：郭姣梅 郝建华[1] 李晓涵 王伟[1] 孙晶晶[1] 宋凯 GUO Jiaomei;HAO Jianhua;LI Xiaohan;WANG Wei;SUN Jingjing;SONG Kai(Key Laboratory of Polar Fisheries Development, Ministry of Agriculture and Rural Affairs,Qingdao National Laboratory for Marine Science and Technology Pilot Laboratory forMarine Drugs and Biological Products,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Qingdao 266071, China;School of Food Science, Shanghai Ocean University, Shanghai 201306, China)

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,农业农村部极地渔业开发重点实验室,山东青岛266071 [2]上海海洋大学食品学院,上海201306

出　　处：《功能材料》2020年第7期7190-7195,共6页Journal of Functional Materials

基　　金：国家重点研发计划资助项目(2018YFC0311106);中国水产科学研究院基本科研业务费资助项目(2020TD67);山东省重点研发计划资助项目(2019GHY112030);青岛市市南区科技发展计划资助项目(2018-4-002-ZH)。

摘　　要：采用化学共沉淀法制备Fe3O4纳米粒子,并依次以正硅酸四乙酯(TEOS)为硅源、以3-氨丙基三乙氧基硅烷(APTES)为硅烷偶联剂对磁性纳米粒子进行表面修饰,最终得到氨基硅烷化的磁性纳米粒子(Fe3O4@SiO2-NH2)。采用透射电子显微镜(TEM)、扫描电子显微镜(SEM)、傅里叶红外光谱分析仪(FTIR)、X射线衍射(XRD)、振动样品磁强计(VSM)和热重分析仪(TGA)对磁性纳米粒子的形态、结构进行表征。结果表明,氨基硅烷化的磁性纳米粒子具有良好的超顺磁性,结晶度高,稳定性好,粒径约为22 nm。利用该磁性材料固定化海洋环糊精葡萄糖基转移酶(CGTase)得,固定化酶热稳定性,对酸碱的耐受性和储存稳定性明显优于游离酶。In this study,magnetic Fe3O4 nanoparticles were synthesized by co-precipitation method,and amino silanized magnetic nanoparticles(Fe3O4@SiO2-NH2)were obtained through adding tetraethyl orthosilicate(TEOS)as the silicon source and 3-aminopropyltriethoxysilane(APTES)as the silane coupling agent into Fe3O4 nanoparticles.The morphology and structure of magnetic nanoparticles were characterized by transmission electron microscope(TEM),scanning electron microscope(SEM),Fourier infrared spectrometer(FTIR),X-ray diffraction(XRD),vibration sample magnetometer(VSM)and thermogravimetric analyzer(TGA).The results show that the amino silanized magnetic nanoparticles had good superparamagnetism,high crystallinity,and good stability with the particle size of about 22 nm.It shows that immobilized enzyme were significantly better than free enzyme in thermal stability,resistance to acid and alkali and storage stability,when using the magnetic material to immobilize marine cyclodextrin glucosyltransferase(CGTase).

关 键 词：磁性材料 FE3O4 氨基硅烷化 环糊精葡萄糖基转移酶 固定化 

分 类 号：TM271[一般工业技术—材料科学与工程] Q814.2[电气工程—电工理论与新技术]