具有高细胞黏附性及高生物膜形成能力的植物乳杆菌有效抑制小鼠体内空肠弯曲杆菌毒力因子的转录活性  

Lactobacillus plantarum with high cell adhesion and biofilm formation ability effectively inhibits the transcriptional activities of Campylobacter jejuni virulence genes in mice

作　　者：金星[1] 贺禹丰 周永华 陈晓华 王刚[1] 赵建新[1] 张灏[1] 陈卫[1] JIN Xing;HE Yufeng;ZHOU Yonghua;CHEN Xiaohua;WANG Gang;ZHAO Jianxin;ZHANG Hao;CHEN Wei(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Key Laboratory of Parasitic Disease Prevention and Control Technology for National Health and Health Commission,Wuxi 214064,China;College of Life Science and Environment,Hengyang Normal University,Hengyang 421008,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]国家卫生健康委寄生虫病预防与控制技术重点实验室,江苏无锡214064 [3]衡阳师范学院生命科学与环境学院,湖南衡阳421008

出　　处：《食品与发酵工业》2020年第14期12-18,共7页Food and Fermentation Industries

基　　金：国家自然科学基金资助项目(31671839,31601444,31301407);湖南省教育厅资助科研项目(15B034);湖南省自然科学基金资助项目(2019JJ50014)。

摘　　要：该研究考察了12株植物乳杆菌(Lactobacillus plantarum)的生物学特性,包括其生长速率、产酸能力、对HT-29细胞的黏附能力、形成生物膜的能力、表面疏水性以及耐酸耐胆盐的能力,同时,将这12株植物乳杆菌分别干预被空肠弯曲杆菌(Campylobacter jejuni)和弓形虫复合感染的小鼠并考察其对宿主体内空肠弯曲杆菌毒力基因转录水平的影响。结果显示,植物乳杆菌N36和ZL4可以显著降低小鼠体内空肠弯曲杆菌毒力基因的转录水平。将植物乳杆菌生物学特性和其干预后小鼠体内空肠弯曲杆菌毒力基因转录变化进行相关性分析后发现,菌体表面高疏水性所带来的高细胞黏附性及较强的菌株生物膜形成能力使得其能在小鼠体内显著降低空肠弯曲杆菌毒力基因的转录水平,抑制空肠弯曲杆菌的感染。In this study,the biological characteristics of 12 Lactobacillus plantarum strains were evaluated,including their growth rate,ability of acid production,adhesion to HT-29 cells,ability to form biofilms,surface hydrophobicity,and acid and bile salt resistance. Meanwhile,these strains were administered to Campylobacter jejuni and Toxoplasma gondii co-infected mice by gavage. The results showed that Lactobacillus plantarum N36 and ZL4 could significantly reduce the transcriptional activities of Campylobacter jejuni virulence genes. The correlation analysis of biological characteristics of Lactobacillus plantarum and the transcriptional activities of Campylobacter jejuni virulence genes showed that the high cell adhesion caused by the high surface hydrophobicity on bacterial body and the strong biofilm formation ability of the strains could significantly reduce the virulence genes transcriptional activities of Campylobacter jejuni in mice.

关 键 词：植物乳杆菌 空肠弯曲杆菌 毒力基因 

分 类 号：TS201.3[轻工技术与工程—食品科学]