环境对蜂房哈夫尼亚菌群体感应基因haII/haIR表达的影响  

Effects of Environments on Gene haII/haIR Expression of Hafnia alvei Based on Quorum Sensing System

作　　者：赫彬彬 李婷婷[2] 梅永超 王当丰 孙晓佳 刘楠 励建荣 He Binbin;Li Tingting;Mei Yongchao;Wang Dangfeng;Sun Xiaojia;Liu Nan;Li Jianrong(Food Science Research Institute of Bohai University,Food Safety Key Lab of Liaoning Province,Jinzhou 121013,Liaoning;Key Laboratory of Biotechnology and Bioresources Utilization(Dalian Minzu University),Ministry of Education,Dalian 116600,Liaoning)

机构地区：[1]渤海大学食品科学与工程学院辽宁省食品安全重点实验室,辽宁锦州121013 [2]生物技术与资源利用教育部重点实验室(大连民族大学),辽宁大连116600

出　　处：《中国食品学报》2020年第7期10-17,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31301572,31301418)。

摘　　要：以蜂房哈夫尼亚菌群体感应基因haII/haIR为对象,以16S rRNA为内参基因,采用实时荧光定量PCR技术探究不同环境对感应基因haII/haIR表达的影响,以明晰环境因子对群体感应稳定的影响及其机制。结果表明:不同碳源培养条件下,蜂房哈夫尼亚菌haII基因表达情况依次为木糖>果糖>葡萄糖>麦芽糖>乳糖>蔗糖;haIR基因表达情况为木糖>果糖>蔗糖>葡萄糖>乳糖>麦芽糖;当环境中氯化钠浓度较高时,两个基因表达均受到抑制。不同pH培养条件下,haII/haIR基因在酸性条件下表达情况较好。低温(15℃)培养时,基因表达量明显上调;而超过其最适温度,表达量又有所下降。本研究结果为对丰富群体感应理论,拓展群体感应理论在水产品保鲜中的应用具有重要意义。Quorum sensing haII/haIR gene was used for this study and 16 S rRNA was used as the reference gene,the expression of quorum sensing haII/haIR gene in different environments was determined by real-time fluorescence quantitative PCR to explore the mechanism of different environmental factors on quorum sensing stability.The results showed that the haII gene expression under different carbon source culture conditions was xylose > fructose > glucose >maltose> lactose> sucrose,and the expression of haIR gene was xylose > fructose > sucrose > glucose > lactose > maltose.The expression of haII/haIR gene were inhibited as sodium chloride concentration increased,and haII/haIR genes could be expressed under acid conditions.At the same time,genes expression had a significant up-regulation at 15 ℃,however beyond the optimum temperature,the expression decreased.Our results had an important implication for enriching the quorum sensing theory and expanding application of quorum sensing theory in the preservation of aquatic production.

关 键 词：蜂房哈夫尼亚菌 群体感应基因 实时荧光定量PCR 不同环境因素 

分 类 号：TS201.3[轻工技术与工程—食品科学]