METTL4 is an snRNA m^6Am methyltransferase that regulates RNA splicing  

作　　者：Hao Chen Lei Gu Esteban A.Orellana Yuanyuan Wang Jiaojiao Guo Qi Liu Longfei Wang Zhangfei Shen Hao Wu Richard I.Gregory Yi Xing Yang Shi 

机构地区：[1]Division of Newborn Medicine and Program in Epigenetics,Boston Children's Hospital,Harvard Medical School,300 Longwood Avenue,Boston,MA 02115,USA [2]Stem Cell Program,Division of Hematology/Oncology,Boston Children's Hospital,Deportment of Biological Chemistry and Molecular Pharmacology,Harvard Medical School,Boston,MA 02115,USA [3]Bioinformatics Interdepartmental Graduate Program,University of California,Los Angeles,Los Angeles,CA 90095,USA [4]College of Science,Northeastern University,360 Huntington Ave.,Boston,MA 02115,USA [5]Program in Cellular and Molecular Medicine,Boston Children's Hospital,Boston,MA 02115,USA [6]Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School,Boston,MA 02115,USA [7]Center for Computational and Genomic Medicine,The Children’s Hospital of Philadelphia,Philodelphio,PA 19104,USA [8]Department of Pathology and Laboratory Medicine,University of Pennsylvania,Philadelphia,PA 19104,USA

出　　处：《Cell Research》2020年第6期544-547,共4页细胞研究（英文版）

基　　金：We thank Chunmei Li,Matthew Harris,Erdem Sendinc,and David Valle-Garcia for insightful advice.Y.S.is supported by BCH funds.R.I.G.is a recipient of the NCI Outstanding investigator Award(R35 CA232115-01);E.A.O.was supported by the Pew Latin American Fellows Program and by a Damon Runyon Fellowship(DRG-2378-19);Y.X.is supported by NIH grants(R01GM088342 and R01GM121827);Y.S.is an American Cancer Society Research Professor.

摘　　要：Dear Editor,Protein coding mRNAs and non-coding RNAs are extensively modifed by chemical modifications,'including methylation of the N^6position of adenosine(m^6A).^2While mRNA internal m^6A methylation is mediated by METTL3 and METTL14,which are members of the MT-A70 family,N^6-methylation of cap-adjacent No,2'-O-dimethyladenosine(m^6Am)is catalyzed by PCIF1.^3-6 Non-coding RNAs are also m^6Am methylated internally,7but the corresponding enzyme remains unknown.Here we provide evidence that METTL4 mediates internal m^6Am methylation of U2 small nuclear RNA(snRNA).

关 键 词：METHYLATION MODIFICATION METHYLTRANSFERASE 

分 类 号：Q55[生物学—生物化学]