超高效液相色谱-串联质谱法测定牛奶和奶粉中五种苯并咪唑类药物  

Determination of Five Benzimidazoles in Milk and Milk Powder by UPLC-MS-MS

作　　者：陈瑞 杨志伟 朱凤妹[1] 齐鹏宇 李响 李军[1] 张进杰 CHEN Rui;YANG Zhiwei;ZHU Fengmei;QI Pengyu;LI Xiang;LI Jun;ZHANG Jinjie(College of Food Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066600;Qinhuangdao Customs Technology Center,Qinhuangdao,Hebei 066004)

机构地区：[1]河北科技师范学院食品科技学院,河北秦皇岛066600 [2]秦皇岛海关技术中心,河北秦皇岛066004

出　　处：《核农学报》2020年第8期1776-1784,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家重点研发计划“食品安全关键技术研发”重点专项(2017YFC1601700);国家自然科学基金(31570374、31470542);河北省科技计划项目(17275503D)。

摘　　要：为建立牛奶及奶粉中5种苯并咪唑类药物(阿苯达唑、芬苯达唑、奥芬达唑、噻苯达唑、苯硫氨酯)简便、准确的分析检测方法,以固相萃取为净化手段,采用Acquity UPLC?BEH C18(100 mm×2.1 mm,1.7μm)色谱柱进行分离,电喷雾离子源(ESI+),多反应监测(MRM)模式检测。结果表明,5种苯并咪唑类药物在1~500μg·L^-1范围内呈线性相关,相关系数(r)均大于0.996,加标平均回收率介于91.7%~97.8%之间,相对标准偏差(RSD)在1.4%~6.5%之间,基质效应均小于15%。5种药物的检出限均在0.1~3μg·kg^-1范围内,定量限均在0.5~10μg·kg^-1范围内。该方法操作简单、快速且灵敏度高,重现性好,能够用于牛奶及奶粉5种苯并咪唑类药物的同时测定。To establish a simple and accurate analytical method for the determination of five benzimidazoles(albendazole, fenbendazole, oxfendazole, thiabendazole, phenylthiourethane) in milk and milk powder, solid phase extraction was used as a purification method. Acquity UPLC■ BEH C18(100 mm×2.1 mm, 1.7 μm) column for separation, and electrospray ion source(ESI+) for multiple reaction monitoring(MRM) mode detection. The results indicated that the five benzimidazoles were linearly correlated in the range of 1-500 μg·L^-1 with the correlation coefficient(r) greater than 0.996. The recoveries and RSD were from 91.7%-97.8% and 1.4%-6.5%, respectively. The matrix effects were less than 15%. The LOD and LOQ were in the range of 0.1-3 μg·kg^-1 and 0.5-10 μg·kg^-1, respectively.The method was simple, rapid, sensitive and reproducible, and coulde be used for simultaneous determination of five benzimidazole in milk and milk powder.

关 键 词：牛奶 奶粉 超高效液相色谱-串联质谱(UPLC-MS/MS) 苯并咪唑类药物 

分 类 号：O657.63[理学—分析化学] TS252.7[理学—化学]