基于石墨烯和金纳米棒的电化学核酸适配体传感器同时检测赭曲霉毒素A和伏马菌素B1  

Electrochemical aptasensor based on graphene and gold nanorods for simultaneous detection of ochratoxin A and fumonisin B1

作　　者：梁秀俊 冯硕 卫敏[1] 卫伟[2] LIANG Xiujun;FENG Shuo;WEI Min;WEI Wei(College of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,China;School of Chemistry and Engineering,Southeast University,Nanjing 211189,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001 [2]东南大学化学化工学院,江苏南京211189

出　　处：《生物加工过程》2020年第4期440-445,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家重点研发计划(2018YFC1602801)。

摘　　要：利用石墨烯(GR)通过ππ相互作用吸附单链DNA,结合金纳米棒(AuNRs)对巯基修饰的物质的高吸附能力,构建新型电化学核酸适配体传感器,用于赭曲霉毒素A(OTA)和伏马菌素B1(FB 1)的同时检测研究。GR和AuNRs具有比表面积大、导电性强的优点,能够实现信号放大,电化学信号探针硫堇和二茂铁的初始电流信号分别达到299.3和314.8μA。利用制备的传感器对OTA和FB 1进行同时检测,得到线性范围为5×10^-4~1×10^3 ng/mL,检出限分别为0.34和0.17 pg/mL。该传感器对黄曲霉毒素B1和玉米赤霉烯酮有较好的抗干扰性能。对相同条件下制备的传感器进行测定的结果表明,该传感器具有可接受的重现性和重复性。对OTA和FB 1加标的啤酒样品进行检测,平均回收率分别为94.1%~97.7%和87.1%~95.3%,表明该传感器可以用于实际样品的检测。Based on the interaction of grapheme(GR)and DNA byπ-πstacking,and high adsorption capacity of gold nanorods(AuNRs)for thiol-modified molecules,anovel electrochemical aptamer aptasensor was developed for simultaneous detecting ochratoxin A(OTA)and fumonisin B 1(FB 1).GR and AuNRs have the advantages of large specific surface area and strong conductivity,which can realize signal amplification.The initial signals of thioine and ferrocene reached 299.3 and 314.8μA,respectively.The linear range of OTA and FB 1 was 5×10^-4-1×10^3 ng/mL,and the limit of detection was 0.34 and 0.17 pg/mL,respectively.The aptasensor had good anti-interference performance to aflatoxin B1 and zearalenone.It was applied to detect OTA and FB 1 in spiked beer with the recoveries of 94.1%-97.7%and 87.1%-95.3%,indicating that the prepared aptasensor can be used for the detection of actual samples.

关 键 词：电化学传感器 核酸适配体 石墨烯 金纳米棒 真菌毒素 

分 类 号：O652[理学—分析化学]