益生菌对卵巢摘除致雌激素缺乏小鼠牙周组织中IL-17表达的影响及其意义  

Effect of probiotics on expression of IL-17 in periodontal tissue of estrogen-deficient mice induced by ovariectomy and its significance

作　　者：黄江勇[1] 李婵秀[2] 郑志超 王洪娟 郭吕华[1] 吴哲[1] 罗涛[1] HUANG Jiangyong;LI Chanxiu;ZHENG Zhichao;WANG Hongjuan;GUO Lyuhua;WU Zhe;LUO Tao(Department of Prosthodontics,Affiliated Stomatology Hospital,Guangzhou Medical University,Key Laboratory of Oral Medicine,Guangzhou Institute of Oral Disease,Guangzhou 510140,China;Department of Stomatology,Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510055,China;Shenzhen Health Time Gene Company,Shenzhen 518000,China)

机构地区：[1]广州医科大学附属口腔医院修复科·广州口腔病研究所·口腔医学重点实验室,广东广州510140 [2]中山大学附属第三医院口腔科,广东广州510000 [3]广东省深圳海一时代基因科技有限公司,广东深圳518000

出　　处：《吉林大学学报（医学版）》2020年第4期733-738,共6页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金青年科学基金资助课题(81300902);广东省医学科学技术研究基金资助课题(B2018099)。

摘　　要：目的:探讨益生菌干预对雌激素缺乏小鼠牙周组织中白细胞介素17(IL-17)表达的影响,阐明雌激素缺乏与牙周炎关联性的可能机制。方法:选取8周龄健康雌性C57BL/6J小鼠15只,随机分为假手术组、卵巢摘除组和益生菌干预组(卵巢摘除术后行益生菌灌服),每组5只。采用平均骨密度测量技术检测实验前后小鼠骨矿物质密度(BMD),实时荧光定量PCR(RT-qPCR)法检测小鼠骨髓细胞和牙龈组织中IL-17 mRNA表达水平,流式细胞术检测小鼠骨髓中辅助性T细胞17(Th17)占CD4+TCRβ+细胞的百分率。结果:与假手术组比较,卵巢摘除组小鼠BMD明显降低(P<0.05),骨髓细胞和牙龈组织中IL-17 mRNA表达水平均明显升高(P<0.05),Th17细胞占骨髓CD4+TCRβ+细胞百分率明显升高(P<0.05);与卵巢摘除组比较,益生菌干预组小鼠BMD明显增加(P<0.05),骨髓细胞和牙龈组织中IL-17 mRNA表达水平均明显降低(P<0.05),Th17细胞占骨髓CD4+TCRβ+细胞百分率明显降低(P<0.05);与假手术组比较,益生菌干预组小鼠股骨BMD、骨髓细胞和牙龈组织中IL-17 mRNA表达水平及Th17细胞占骨髓CD4+TCRβ+细胞百分率差异均无统计学意义(P>0.05)。结论:益生菌干预能阻止雌激素缺乏导致的小鼠牙周组织中IL-17水平升高,对雌激素缺乏引起的牙周炎具有预防作用。Objective:To explore the effect of probiotic feeding on the interleukin-17(IL-17)expression in periodontal tissue of the estrogen-deficient mice,and to elucidate the possible mechanism of the correlation between estrogen deficiency and the periodontitis susceptibility.Methods:A total of 15 female C57BL/J6 mice(8-week-old)were randomly divided into sham operation group,ovariectomy group and probiotics group(probiotics feeding after ovariectomy)(n=5).The bone mineral densities(BMD)of the mice in various groups before and after experiment were measured by mean bone density measurement technique and the IL-17 mRNA expression levels in bone marrow cells and gingiva tissus of the mice were determined by RT-qPCR.The percentages of Th17 cells in CD4+TCRβ+B cells in bone marrow of the mice were measured by flow cytometry.Results:Compared with sham operation group,the BMD of femur of the mice in OVX group was significantly decreased(P<0.01),the expression levels of IL-17 mRNA in bone marrow ce lls and gingiva tissue were increased(P<0.01),and the percentage of CD4+TCRβ+Th17 cells in the bone marrow was increased(P<0.05).Compared with OVX group,the BMD of femur of the mice in probiotics group was significantly increased(P<0.01),the expression levels of IL-17 mRNA in bone marrow cells and gingiva tissue were decreased(P<0.01),and the percentage of CD4+TCRβ+Th17 cells in the bone marrow was decreased(P<0.05).Compared with sham operation group,the BMD of femur,the expression levels of IL-17 mRNA in bone marrow cells and gingiva tissue,and the percentage of CD4+TCRβ+Th17 cells in the bone marrow in probiotics group had no significant differences(P>0.05).Conclusion:Probiotics feeding can inhibit the upregulation of IL-17 mRNA level of the mice induced by estrogen deficiency,and has the preventive effect on the estrogen deficiency-induced periodontitis.

关 键 词：雌激素缺乏 益生菌 白细胞介素17 辅助性T细胞17 牙周炎 

分 类 号：R781.4[医药卫生—口腔医学] R-332[医药卫生—临床医学]