响应面法优选芜菁中蛋白质的提取工艺及蛋白质抗氧化活性的评估  

Optimization of the extraction process of Brassica rapa L.protein by response surface methodology and evaluation of its antioxidant activity

作　　者：胡尔西丹·伊麻木[1] 李亚童 乔丽洁 莫罕美竟也 海力茜·陶尔大洪[1] 杨飞 HUERXIDAN Yi-Ma-Mu;LI Ya-Tong;QIAO Li-Jie;MOHANMEIJINGYE;HAILIQIAN Tao-Er-Da-Hong;YANG Fei(School of Pharmacy,Xinjiang Medical University,Urumqi 830011,China;School of Business,University of Southampton,Southampton SO171BJ,United Kingdom;Xinjiang Uygur Autonomous Region Institute of Analysis and Testing,Urumqi 830011,China)

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011 [2]南安普顿大学商学院,南安普顿SO171BJ [3]新疆维吾尔自治区分析测试研究院,乌鲁木齐830011

出　　处：《食品安全质量检测学报》2020年第13期4482-4488,共7页Journal of Food Safety and Quality

基　　金：新疆维吾尔自治区科技厅项目(2017D01C202);自治区“十三五”重点学科建设经费支持(1007)。

摘　　要：目的借助单因素实验和响应面法优选芜菁中蛋白质的提取工艺,并评估芜菁蛋白质的抗氧化活性。方法采用超声辅助提取芜菁中蛋白质。通过单因素实验,选取实验因素与水平,分别研究了料液比、超声功率、超声提取时间、提取温度对蛋白提取量的影响。根据Box-Benhnken中心组合实验设计原理,采用3因素3水平的响应面分析法,对蛋白质的提取工艺进行优化,同时通过比较芜菁蛋白质与维生素C对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基的清除能力,评估芜菁蛋白质抗氧化活性。结果优化后的芜菁蛋白提取工艺条件为:料液比1:18.88(m/V),超声功率120 W,超声提取时间6.12 min。当芜菁蛋白质溶液质量浓度为25 mg/mL时,对DPPH自由基的清除率为99.3%。在此条件下得出的芜菁的蛋白质含量为21.71 mg/g,与预测值相近。结论在最优条件下,芜菁中蛋白质的提取率得到了提高。其对DPPH的清除能力随其质量浓度的增大而增强;其清除能力弱于抗坏血酸。Objective To optimize the extraction process of protein from Brassica rapa L.by single factor experiment and response surface method,and evaluate the antioxidant activity of the protein.Methods The protein from Brassica rapa L.was extracted through ultrasound-assisted method.Experiment factors and levels were selected by one-factor tests and the effects of solid-liquid ratio,ultrasonic power,ultrasonic extraction time,and extraction temperature on the extraction of protein were studied.According to the Box-Benhnken center-united experimental design principles,the method of response surface analysis with 3 factors and 3 levels was adopted to optimize the extraction technology.Meanwhile,the antioxidant capacity of the protein from Brassica rapa L.was evaluated.Results The optimum extraction conditions by response surface method were as follow:solid-liquid ratio 1:18.88(m/V),ultrasonic power 120 W,ultrasonic extraction time 6.12 min.When the protein mass concentration was 25 mg/mL,the scavenging rate on DPPH free radical was 99.3%.The total protein content of the Brassica rapa L.obtained under the conditions was 21.71 mg/g,similar to the predictedvalue.Conclusions Under the optimal conditions,the extraction rate of protein from Brassica rapa L.is improved.The scavenging capacity on DPPH free radical increases along with the increase of the protein concentration,which is inferior to that of ascorbic acid.

关 键 词：芜菁 蛋白质 响应面优化 单因素实验 抗氧化活性 

分 类 号：TQ936[化学工程]