贵州羊肚菌的分子系统发育分析  

Molecular Phylogenetic Analysis of Morchella in Guizhou

作　　者：李银双 刘作易[4] 谭玉梅 任锡毅[2,3] 刘永翔 黄永会[2,3] LI Yinshuang;LIU Zuoyi;TAN Yumei;REN Xiyi;LIU Yongxiang;HUANG Yonghui(College of Life Sciences,Guizhou University,Guiyung,Guizhou 550025,China;Institute of Biotechnology,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,China;Guizhou Key Laboratory of Agricultural Biotechnology,Guiyang,Guizhou 550006,China;Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,China)

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省农业科学院生物技术研究所,贵州贵阳550006 [3]贵州省农业生物技术重点实验室,贵州贵阳550006 [4]贵州省农业科学院,贵州贵阳550006

出　　处：《山地农业生物学报》2020年第3期52-60,共9页Journal of Mountain Agriculture and Biology

基　　金：西南喀斯特地区特色微生物资源及多样性调查(项目编号:2014FY120100)。

摘　　要：本研究以贵州省贵阳市、六盘水、遵义和铜仁等地采集的羊肚菌样本中挑选出24份子实体样本,采用五个基因片段(ITS、LSU、EF1-a、RPB1、RPB2)联合分析,结合子实体形态特征对其进行了物种鉴定。研究结果表明:贵州省境内共分布有8个野生羊肚菌物种,包括黑色羊肚菌和黄色羊肚菌两个类群,黑色羊肚菌有梯棱羊肚菌(M.importuna)、Mel-19、Mel-21,黄色羊肚菌有Mes-15、Mes-19、Mes-22、Mes-23、Mes-27。野生羊肚菌的发生时间为3—5月,具有喜湿喜阴的生长特性,Mes-15是首次报道具有火烧地偏好性的黄色种。Combined with morphological characteristics of fruiting body of Morchella,24 samples collected from Guiyang,Liupanshui,Zunyi and Tongren regions,Guizhou Province were identified using five gene fragments(ITS,LSU,EF1-a,RPB1,RPB2).The results showed that there existed 8 wild Morchella species in Guizhou Province,including two groups of black and yellow Morchella.Black morel are M.importuna,Mel-19,Mel-21,and yellow one containing Mes-15,Mes-19,Mes-22,Mes-23,Mes-27.The occurrence of wild morels in Guizhou was from March to May and the species prefered moisture and shade.Mes-15 was first reported to prefer to growing in burnt land.

关 键 词：贵州 野生羊肚菌 系统发育 多基因联合 

分 类 号：Q-9[生物学]