柑普提取物对HepG2和SGC-7901肿瘤细胞系的抗增殖作用  

The Antiproliferative Activities in Ganpu Tea on HepG2 and SGC-7901 Tumor Cell Lines

作　　者：俞乐安 肖遂[2] 黄亚辉[1] 李小晓 赖惠清 郑意 梁大华 张旭[2] YU Le-an;XIAO Sui;HUANG Ya-hui;LI Xiao-xiao;LAI Hui-qing;ZHENG Yi;LIANG Da-hua;ZHANG Xu(College of Horticulture,South China Agricultural University,Guangzhou 510642,China;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China;Yundingganpu Tea Company,Guangzhou 510642,China)

机构地区：[1]华南农业大学园艺学院,广东广州510642 [2]华南农业大学林学与风景学院,广东广州510642 [3]广州市云鼎柑普茶业有限公司,广东广州510642

出　　处：《现代食品科技》2020年第7期42-49,共8页Modern Food Science and Technology

基　　金：广东省茶叶产业体系(2019LM1117)。

摘　　要：本实验研究了柑普茶加工后的成分变化以及对Hep G2与胃癌细胞SGC-7901细胞的抗增殖作用。采用高效液相色谱法对柑普茶品质成分进行了鉴定;以肿瘤细胞Hep G2与SGC-7901为模型,测定了柑普茶及其原料茶抗增殖活性(IC50),对效果较明显的Hep G2细胞系进行了进一步的细胞周期与凋亡分析。结果表明,柑普茶加工后,多酚类物质含量为17.22%,较原料茶显著降低,其中,酯型儿茶素与非酯型儿茶素含量降低显著,而以茶黄素、茶红素为代表的茶色素含量则有所提高。柑普茶在抑制SGC-7901与Hep G2细胞体外增值方面的作用明显强于普洱茶和柑橘皮,其IC50分别为176.61μg/m L与143.60μg/m L。通过对Hep G2细胞周期与凋亡分析,表明柑普茶对肝癌细胞系的抑制作用主要通过阻滞细胞周期在G0/G1期后进而诱导细胞凋亡引起。柑普茶特殊加工工艺有利于其风味改善,也有利于活性物质积累,使得其在抑制Hep G2与SGC-7901肿瘤细胞增殖方面表现出更好的活性。In this study, the changes of components in the processed ganpu tea and their antiproliferative effects on HepG2 and SGC-7901 cells were investigated. The change of quality components in ganpu tea was determined by high performance liquid chromatography, and the activity of ganpu tea extracts against HepG2 and SGC-7901 colonies was determined by the MTT method. Then, the cell cycle and apoptosis of Hep G2 cell line with obvious effect were further analyzed. The results showed that after the processing, the content of polyphenols was 17.22% which was lower than the material tea. Among them, the ester catechins and non-ester catechins were remarkably reduced. Moreover, the increase of tea pigments(theaflavins and thearubigins) was prominent. Ganpu tea had a higher tumor inhibition rate on SGC-7901 and HepG2 cell lines than puer tea and citrus peel, and the IC50 values were up to 176.61 μg/m L and 143.60 μg/m L, respectively. The analysis of cell cycle and apoptosis indicated that ganpu tea induced cell apoptosis through retarding the G0/G1 in HepG2 cells. As a result, the special processing of ganpu tea not only prompted the amelioration of flavor but also improved the accumulation of active substances so that ganpu tea showed better antiproliferative activities in HepG2 and SGC-7901 tumor cell lines.

关 键 词：柑普茶 品质成分 抗增殖 肿瘤 细胞系 

分 类 号：TS272.5[农业科学—茶叶生产加工] R285.5[轻工技术与工程—农产品加工及贮藏工程]