Dysregulation of microRNA in cholangiocarcinoma identified through a meta-analysis of microRNA profiling  

作　　者：Somsak Likhitrattanapisal Supeecha Kumkate Pravech Ajawatanawong Kanokpan Wongprasert Rutaiwan Tohtong Tavan Janvilisri 

机构地区：[1]National Center for Genetic Engineering and Biotechnology,Pathumthani 12120,Thailand [2]Department of Biology,Faculty of Science,Mahidol University,Bangkok 10400,Thailand [3]Division of Bioinformatics and Data Management for Research,Faculty of Medicine Siriraj Hospital,Mahidol University,Bangkok 10700,Thailand [4]Department of Anatomy,Faculty of Science,Mahidol University,Bangkok 10400,Thailand [5]Department of Biochemistry,Faculty of Science,Mahidol University,Bangkok 10400,Thailand

出　　处：《World Journal of Gastroenterology》2020年第29期4356-4371,共16页世界胃肠病学杂志（英文版）

基　　金：Supported by the Thailand Research Fund,No.DBG5980006;UK-Thailand Research Collaborations(Newton Fund),No.MR/N01247X/1.

摘　　要：BACKGROUND In the past decades,the potential of microRNA(miRNA)in cancer diagnostics and prognostics has gained a lot of interests.In this study,a meta-analysis was conducted upon the pooled miRNA microarray data of cholangiocarcinoma(CCA).AIM To identify differentially expressed(DE)miRNAs and perform functional analyses in order to gain insights to understanding miRNA-target interactions involved in tumorigenesis pathways of CCA.METHODS Raw data from 8 CCA miRNA microarray datasets,consisting of 443 samples in total,were integrated and statistically analyzed to identify DE miRNAs via comparison of levels of miRNA expression between CCA and normal bile duct samples using t-tests(P<0.001).The 10-fold cross validation was performed in order to increase the robustness of the t-test results.Our data showed 70 up-regulated and 48 down-regulated miRNAs in CCA. GeneOntology and pathway enrichment analyses revealed that mRNA targets of DEmiRNAs were significantly involved in several biological processes. The mostprominent dysregulated pathways included phosphatidylinositol-3 kinases/Akt,mitogen-activated protein kinase and Ras signaling pathways.CONCLUSIONDE miRNAs found in our meta-analysis revealed dysregulation in major cancerpathways involved in the development of CCA. These results indicated thenecessity of understanding the miRNA-target interactions and the significance ofdysregulated miRNAs in terms of diagnostics and prognostics of cancers.

关 键 词：CHOLANGIOCARCINOMA MICROARRAY MICRORNA META-ANALYSIS 

分 类 号：R735.8[医药卫生—肿瘤]