仙茅不同炮制品对腺嘌呤致肾阳虚大鼠的作用机制分析  

Analysis on Mechanism of Adenine-induced Kidney-Yang Deficiency Rats Treated with Different Processed Products of Curculiginis Rhizoma

作　　者：鞠成国[1] 李媛媛 王巍[1] 贾天柱[1] 艾雪[1] JU Cheng-guo;LI Yuan-yuan;WANG Wei;JIA Tian-zhu;AI Xue(School of Pharmacy,Chinese Materia Medica Processing Engineering Center of Liaoning Province,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院辽宁省中药炮制工程技术研究中心,辽宁大连116600

出　　处：《中国实验方剂学杂志》2020年第16期101-107,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省自然科学基金项目(20180550241);2019年全国中药特色技术传承人才培训项目(T20194828003)。

摘　　要：目的:研究仙茅不同炮制品水提液对腺嘌呤所致肾阳虚大鼠的补肾壮阳作用机制。方法:以桂附地黄丸为阳性组(给药剂量2. 466 g·kg^-1),采用酶联免疫吸附测定法(ELISA)比较仙茅不同炮制品水提液(给药剂量2. 742 g·kg^-1)灌胃4周后对腺嘌呤所致肾阳虚大鼠血清中促甲状腺激素(TSH),雌二醇(E2),睾酮(T),17-羟皮质类固醇(17-OHCS),三碘甲状腺原氨酸(T3),甲状腺素(T4),皮质醇(COR)水平的影响;采用Nash比色法测定各组大鼠肝、肾微粒体细胞色素P450 3A(CYP3A)的活性。结果:仙茅各炮制品水提液对腺嘌呤所致肾阳虚大鼠的下丘脑-垂体-靶腺轴功能均有一定程度的改善作用,各炮制品总评分排序为萸仙茅(29分)>盐仙茅(25分)>酒仙茅(23分)>生仙茅(17分)>姜仙茅(11分);仙茅各炮制品组均能不同程度上调肾阳虚状态下大鼠肝、肾微粒体CYP3A活性,其中盐仙茅和萸仙茅效果较优。结论:仙茅各炮制品均能有效治疗大鼠肾阳虚证,其中萸仙茅、盐仙茅补肾壮阳效果较为显著。Objective: To study on the mechanism of invigorating kidney and strengthening Yang of different processed products of Curculiginis Rhizoma aqueous extracts in rats with kidney-Yang deficiency induced by adenine. Method: Taking Guifu Dihuang pills as the positive drug group(the dosage of 2. 466 g·kg^-1),after intragastric administration for 4 weeks,enzyme-linked immunosorbent assay(ELISA)was used to compare the effects of different processed products of Curculiginis Rhizoma aqueous extracts(the dosage of2. 742 g·kg^-1)on the levels of thyroid stimulating hormone(TSH),17-hydroxycorticosteroids(17-OHCS),estradiol(E2),testosterone(T),triiodothyronine(T3),thyroxine(T4)and cortisol(COR)in serum of rats with kidney-Yang deficiency induced by adenine. The activity of cytochrome P450 3 A(CYP3 A)in rat liver and kidney microsomes was determined by Nash colorimetry. Result: All the processed products aqueous extracts could improve the function of hypothalamus-pituitary-target gland axis in rats with kidney-Yang deficiency induced by adenine,and the total score was in the order of Euodiae Fructus juice-processed products(29 points)>salt-processed products(25 points)>rice wine-processed products(23 points)>raw products(17 points)>Zingiberis Rhizoma juice-processed products(11 points). And the different processed products of Curculiginis Rhizoma could increase CYP3 A activity of liver and kidney microsomes of kidney-Yang deficiency rats,especially the Euodiae Fructus juice-processed products and salt-processed products. Conclusion: All the processed products of Curculiginis Rhizoma can effectively treat the syndrome of kidney-Yang deficiency in rats,among them,Euodiae Fructus juice-processed products and salt-processed products have more significant effect on invigorating kidney and strengthening Yang.

关 键 词：仙茅 炮制 腺嘌呤 补肾壮阳 细胞色素P4503A(CYP3A) 肾阳虚 下丘脑-垂体-靶腺轴 

分 类 号：R22[医药卫生—中医基础理论] R28[医药卫生—中医学]