β-木糖苷酶的生物活性物质转化功能研究进展  

Research progress in bioactive substances transformation by β-xylosidases

作　　者：李娜 张蕊[1,2,3] 黄遵锡[1,2,3] 周峻沛 LI Na;ZHANG Rui;HUANG Zun-Xi;ZHOU Jun-Pei(School of Life Sciences,Yunnan Normal University,Kunming,Yunnan 650500,China;Engineering Research Center of Sustainable Development and Utilization of Biomass Energy,Ministry of Education,Kunming,Yunnan 650500,China;Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment,Kunming,Yunnan 650500,China)

机构地区：[1]云南师范大学生命科学学院,云南昆明650500 [2]生物能源持续开发利用教育部工程研究中心,云南昆明650500 [3]云南省生物质能与环境生物技术重点实验室,云南昆明650500

出　　处：《微生物学通报》2020年第7期2290-2299,共10页Microbiology China

基　　金：国家自然科学基金(31960459);云南省基础研究计划重点项目(2020-2023:24);云南省万人计划青年拔尖人才项目(YNWR-QNBJ-2018-383);云南师范大学联大青年学者培养计划(01200205020516036)。

摘　　要：β-木糖苷酶是木聚糖酶酶系的一种酶,其功能主要是降解半纤维素中最常见及含量最高的组分——木聚糖。近些年,研究人员发现一些微生物来源的β-木糖苷酶具有生物活性物质转化功能,可通过转糖基作用形成带有木糖基的生物活性物质,也可通过水解作用将带有木糖基的物质,如三七皂苷R1和R2、黄芪甲苷IV(astragaloside IV,ASI)、7-木糖-10-去乙酰紫杉醇(7-xylosyl-10-deacetyltaxol,XDT)和花青素转化为生物活性物质,因此,这些β-木糖苷酶在食品和医药等领域具有巨大的潜在应用价值。此外,研究人员揭示了β-木糖苷酶在生物活性物质转化功能方面的一些机制。本文主要介绍了β-木糖苷酶的生物活性物质转化功能、酶来源、家族分类、转化机制及应用,以期为β-木糖苷酶的进一步开发利用提供参考。β-xylosidases are the members of xylanases.Their functions have been recognized to degrade xylan that is the most common hemicellulosic polysaccharide.In recent years,some microbialβ-xylosidases show the function of bioactive substances transformation,including transxylosylation and dexylosylation of xylosylated substances such as notoginsenosides R1 and R2,astragaloside IV,7-xylosyl-10-deacetylpaclitaxel and anthocyanins to produce bioactive substances.Theseβ-xylosidases have huge potential applications in food,pharmaceutical and many other industries.Furthermore,some mechanisms involved in bioactive substances transformation byβ-xylosidases have been revealed.This review mainly introduces the bioactive substances transformation functions,sources,family classification,transformation mechanisms and applications ofβ-xylosidases.The aim of the review is to provide useful information for the further development and utilization ofβ-xylosidases.

关 键 词：Β-木糖苷酶 生物活性物质 转糖基 转化机制 

分 类 号：Q55[生物学—生物化学]