超声辅助纤维素酶提取青龙衣多糖工艺条件优化  

Ultrasonic-assisted Enzymatic Extraction of Polysaccharide from Qinglongyi

作　　者：李娜 赵文婧[1] 燕平梅[1] 武晓英[1] 李会珍 李晓君 LI Na;ZHAO Wenjing;YAN Pingmei;WU Xiaoying;LI Huizhen;LI Xiaojun(Department of Biology,Taiyuan Normal University,Jinzhong,Shanxi 030619,China;School of Chemical Engineering and Technology,North University of China,Taiyuan,Shanxi 030051,China)

机构地区：[1]太原师范学院生物系,山西晋中030619 [2]中北大学化学工程与技术学院,山西太原030051

出　　处：《农产品加工》2020年第14期34-37,41,共5页Farm Products Processing

基　　金：山西省重点研发计划重点项目(201603D21109-1-2);山西省高等学校科技创新项目(2019L0819)。

摘　　要：以青龙衣为原料,利用超声辅助纤维素酶法对青龙衣多糖的提取工艺条件进行优化。探究了粉碎粒度、料液比、纤维素酶添加量、酶解时间、酶解温度和超声功率对青龙衣多糖提取量的影响,在单因素试验基础上,采用Design Expert 10.0.3.1进行试验设计和曲面响应法对最佳多糖提取条件进行优化,构建预测模型的二次多项式回归方程。结果表明,青龙衣多糖提取的最佳工艺条件为料液比1∶23,超声时间44 min,超声温度48℃,酶添加量1.55%。在该条件下青龙衣多糖实际提取量达到9.43±0.31 mg/g。超声辅助纤维素酶法提取青龙衣多糖的工艺条件简便、提取量高,为实际生产提供了理论依据和技术参考。The present paper optimized the ultrasonic-assisted enzymatic extraction technology of polysaccharide from Qinglongyi by response surface methodology and investigated the effects of particle size,liquid-material ration,cellulase amount,ultrasonic time,ultrasonic temperature and ultrasonic power on the yield of Qinglongyi.Based on the single factor experiment,Design Expert 8.0.6 was used to design the experiment and surface response method was used to optimize the optimal extraction conditions of polysaccharides.According to the model regression analysis,the optimum conditions were as follows:material liquid ration 1∶23,ultrasonic time 44 min,ultrasonic temperature 48℃and cellulase amount 1.55%.Under these conditions,the extracting amount of polysaccharide reached 9.43±0.31 mg/g.The ultrasonic-assisted enzymatic extraction of Qinglongyi polysaccharide was simple with high yield,which could provide theoretical basis and technical reference for practical production.

关 键 词：青龙衣 多糖 超声 酶解法 响应面法 

分 类 号：TS254.5[轻工技术与工程—水产品加工及贮藏工程]