大豆油脱臭馏出物酶法甲酯化工艺研究  

Enzymatic methyl esterification of soybean oil deodorized distillate

作　　者：甘欢华 刘钟栋[1] 吴凯 李华[1] 魏国华 GAN Huanhua;LIU Zhongdong;WU Kai;LI Hua;WEI Guohua(School of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,China;Yichun Big Turtle Life Science Co.,Ltd.,Yichun 336000,Jiangxi,China)

机构地区：[1]河南工业大学粮油食品学院,郑州450001 [2]宜春大海龟生命科学有限公司,江西宜春336000

出　　处：《中国油脂》2020年第7期78-81,92,共5页China Oils and Fats

摘　　要：Eversa Transform 2.0脂肪酶价格低、催化活性好,具有微量高效的优点。以Eversa Transform2.0脂肪酶为催化剂,对大豆油脱臭馏出物进行酶法甲酯化工艺研究。通过单因素试验研究了醇酸摩尔比、酶用量、反应温度、反应时间对酯化率的影响,并进行正交试验,对酶法甲酯化工艺进行优化。在最佳工艺条件下,通过放大试验对试验结果进行验证,并采用薄层色谱法对甘油三酯的转化进行分析。结果表明:Eversa Transform 2.0脂肪酶催化大豆油脱臭馏出物甲酯化反应的最佳工艺条件为醇酸摩尔比(甲醇与游离脂肪酸摩尔比)2∶1、酶用量0.3%(相对于大豆油脱臭馏出物的质量)、反应温度35℃、反应时间6 h,在此条件下酯化率达到95%以上,且甘油三酯基本反应完全。The Eversa Transform 2.0 lipase is cheap and the catalytic activity is good,so it has the advantage of micro-high efficiency.The enzymatic methyl esterification process of soybean oil deodorized distillate was studied with Eversa Transform 2.0 lipase as catalyst.The effects of molar ratio of alcohol to acid,enzyme dosage,reaction temperature and reaction time on esterification rate were studied by single factor experiment.The orthogonal experiment was carried out to optimize the enzymatic methyl esterification process.Under the optimal process conditions,the test results were verified by amplification test,and the conversion of triglyceride was analyzed by thin layer chromatography.The results showed that the optimal conditions for the methyl esterification reaction of soybean oil deodorized distillate catalyzed by Eversa Transform 2.0 lipase were obtained as follows:molar ratio of methanol to free fatty acid 2∶1,enzyme dosage 0.3%(relative to the mass of soybean oil deodorized distillate),reaction temperature 35℃,reaction time 6 h.Under these conditions,the esterification rate reached above 95%,and the triglyceride was almost completely reacted.

关 键 词：大豆油脱臭馏出物 脂肪酶 甲酯化 酸价 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ644[轻工技术与工程—食品科学与工程]