糖胺聚糖降解菌株的筛选及鉴定  

Screening and Identification of Glycosaminoglycan Degradation Strains

作　　者：雷曦 周峻沛[1,2,3,4] 黄遵锡 丁琳[1] 张蕊 LEI Xi;ZHOU Jun-pei;HUANG Zun-xi;DING Lin;ZHANG Rui(Schl.of Life Sei.,Yunnan Normal Uni.,Kunming 650500;Engirt.Res.Ctr.of Sustainable Dev't&Util'n of Biomass Energy,Min 1st.of Educ.,Kunming 650500;Key Lab.of Yunnan far Biomass Energy and Biotech,of Environ't,Kunming 650500;Key Lab.of Enzyme Engirt.,Yunnan Normal Uni.,Kunming 650500)

机构地区：[1]云南师范大学生命科学学院,云南昆明650500 [2]生物能源持续开发利用教育部工程中心,云南昆明650500 [3]云南省生物质能与环境生物技术重点实验室,云南昆明650500 [4]云南师范大学酶工程重点实验室,云南昆明650500

出　　处：《微生物学杂志》2020年第3期22-27,共6页Journal of Microbiology

基　　金：国家重点研发计划项目(2017YFB0308401);国家自然科学基金项目(31460694)。

摘　　要：以土壤为材料,用透明质酸和硫酸软骨素为唯一碳源富集分离菌株,通过BSA-乙酸平板显色法及比色定糖法进行筛选。从80份土壤中筛选出13株糖胺聚糖降解活性的菌株并对其进行了16S rDNA测序鉴定。结果表明,筛选到13株糖胺聚糖降解菌株均具有透明质酸酶和硫酸软骨素酶活性;获得8株尚未报导过的产糖胺聚糖降解酶活性菌株。本研究为开发新型的糖胺聚糖降解酶提供参考。In this study,soil was taken as material adopting hyaluronic acid and cartilage sulfate as the sole carbon source to enrich and isolate strains.And glycosaminoglycan degradation strains were screened by BSA-acetic acid plate coloration method and colorimetric method.13 strains with glycosaminoglycan degradation activity were screened from 80 soil samples and sequenced by 16 S rDNA.The results showed that glycosaminoglycan degradation strains had both activities of hyaluronidase and chondroitinase.The obtained 8 novel strains among them have not been reported to produce glycosaminoglycan degrading enzyme so far.The study provided a foundation to develop novel glycosaminoglycan degradation enzymes.

关 键 词：糖胺聚糖降解酶(Glycosaminoglycan degrading enzyme) 透明质酸 硫酸软骨素 菌株筛选 16S rDNA 

分 类 号：Q93-331[生物学—微生物学]