3,5-二硝基水杨酸法测定液体糖中总糖含量  

Determination of the total sugar content in liquid sugar by 3,5-dinitrosalicylic acid method

作　　者：詹梦涛 娄水珠 刘仙花 洪耀强 杨海英 杜刚[1] ZHAN Meng-tao;LOU Shui-zhu;LIU Xian-hua;HONG Yao-qiang;YANG Hai-ying;DU Gang(Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission and Ministry of Education,Kunming 650500,China;School of Chemistry and Environment,Yunnan Minzu University,Kunming 650500,China)

机构地区：[1]云南民族大学民族药资源化学国家民族事务委员会-教育部重点实验室,云南昆明650500 [2]云南民族大学化学与环境学院,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2020年第4期317-321,共5页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(81860624).

摘　　要：建立3,5-二硝基水杨酸(DNS)测定液体糖中总糖的检测方法,并对总糖的水解条件和还原糖的检测条件进行了优化.结果表明,液体糖中总糖的水解条件为:糖液样品与2 mol/L盐酸体积比为1∶0.5,50℃水浴加热5 min;还原糖的检测条件为:测定波长500 nm,显色剂DNS用量1.5 mL,沸水浴加热7 min,显色时间30 min.标准品葡萄糖在0.1~1.4 mg/mL范围内呈良好的线性关系,平均加标回收率为99.56%,RSD值为3.16%,相关系数R^2=0.999.精密度、重复性和稳定性试验结果的相对标准偏差均小于3%,表明该方法可用于液体糖中总糖含量的测定.The content of total sugar in liquid sugar was determined by 3,5-dinitrosalicylic acid(DNS) method, the hydrolysis conditions of total sugar and the detection conditions of reducing sugar were optimized. The results showed that the hydrolysis conditions of the total sugar were as follows: the ratio of sugar liquid to 2 mol/L hydrochloric acid was 1∶0.5, with 50 ℃water bath heating 5 min;the detection conditions of reducing sugar in liquid sugar were as follows: the absorption wavelength 500 nm, DNS color developing agent 1.5 mL, the boiling water bath 7 min, and the color developing time 30 min. The glucose in the range of 0.1~1.4 mg/mL(R^2=0.999) was in a good linear relationship, and the recovery rate was 99.56%(RSD=3.16%). The relative standard deviation of repeatability, and the results of precision and stability tests were all less than 3%. The DNS method can be used to determine the total sugar in liquid sugar.

关 键 词：3 5-二硝基水杨酸法 液体糖 总糖 测定 

分 类 号：O6573[理学—分析化学] TS247[理学—化学]