武夷茶叶咖啡因含量研究及其聚类分析  

Study on the caffeine content of Wuyi Rock Tea and its cluster analysis

作　　者：金晓怀 林志銮[2] 张传海[3] 马春华 任宇红 武广珩 JIN Xiao-huai;LIN Zhi-luan;ZHANG Chuan-hai;MA Chun-hua;REN Yu-hong;WU Guang-heng(Yushan Health Management School,Wuyi University,Nanping 354300,China;Analysis and Testing Center,Wuyi University,Nanping 354300,China;Fujian Provincial Key Laboratory of Eco-Industrial Green Technology,Wuyi University,Nanping 354300,China;School of Tea and Food Science,Wuyi University,Nanping 354300,China;School of Biological Engineering,East China University of Technology,Shanghai 200237,China)

机构地区：[1]武夷学院玉山健康管理学院,福建南平354300 [2]武夷学院分析测试中心,福建南平354300 [3]武夷学院福建省生态产业绿色技术重点实验室,福建南平354300 [4]武夷学院茶与食品学院,武夷山354300 [5]华东理工大学生物工程学院,上海200237

出　　处：《云南民族大学学报（自然科学版）》2020年第4期322-327,共6页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：福建省科技厅项目(WYKF2017-8,2018L3018,2017Y0076);武夷学院引进人才基金(YT201705).

摘　　要：建立高效液相色谱法测定武夷岩茶中不同产地、加工工艺过程中茶叶的咖啡因含量,采用Agilent Tc C18柱(4.6 mm×150mm,5μm);流动相:乙腈和水;流速:1.000 mL/min;柱温:30℃;检测波长:274 nm;进样量:10μL进行测定.并讨论了溶剂种类、提取时间、提取功率、净化剂选择等因素对咖啡因的影响.实验结果表明:采用甲醇作为溶剂、超声波时间为25 min,提取功率720 W,采用无水Na2SO4和大孔树脂作为净化剂为提取茶叶中的咖啡因最佳提取条件,分析8个样品得知在不同产地、加工工艺过程中茶叶中咖啡因成分在含量上具有较大差异,且具有明显的地域特征.以茶叶中咖啡因含量变化为指标,可以作为区分武夷岩茶产地武夷岩茶品质之一的指标.HPLC was established to determine the caffeine content of Wuyi Rock Tea from different origins. The Aguilent TC C18(4.6 mm × 150 mm, 5 μm) was used. The mobile phase included acetonitrile and water, and the flow rate was 1.000 mL/min;the column temperature was 30 ℃;the detection wavelength was 274 nm, and the sample feed was 10 μL for determination. The effects of the solvent type, extraction time, extraction power and purification agent selection on caffeine were discussed. The experimental results showed that methanol was used as a solvent, the ultrasonic time was 25 min, the extraction power was 720 W, and anhydrous Na2SO4 and macroporous resin were used as purifiers to extract the best conditions for the extraction of caffeine in tea leaves. The analysis of 48 samples showed that there were significant differences in the content of caffeine in teas in different producing areas and processing processes, and there were obvious regional characteristics. With the change of caffeine content in Wuyi Rock Tea as the index, it can be one of the effective factors to distinguish the origin of Wuyi Rock Tea.

关 键 词：武夷岩茶 咖啡因 高效液相色谱法 产地 

分 类 号：O657.72[理学—分析化学] TS275.2[理学—化学]