清旨宁胶囊中沙棘成分薄层色谱鉴别方法  

The TLC Methodology of Sea Buckthorn in Qingzhining Capsules

作　　者：邓辰辰 许明君[1] 相继芬 王婧[1] 丁艳 柏伟荣[1] DENG Chenchen;XU Mingjun;XIANG Jifen;WANG Jing;DING Yan;BAI Weirong(Jiangsu Kanion Parmaceutical Co.,Ltd.,Lianyungang 222047;The Key Laboratory for the New Technique Research of TCM Extraction and Purification,Lianyungang 222047)

机构地区：[1]江苏康缘药业股份有限公司,连云港222047 [2]中药提取精制新技术重点研究室,连云港222047

出　　处：《食品工业》2020年第7期166-170,共5页The Food Industry

基　　金：国家重点研发计划项目“中药肉苁蓉大品种开发与产业化”(2017YFC1702400)。

摘　　要：建立中药复方制剂清旨宁胶囊中沙棘薄层色谱(TLC)鉴别方法。以原料中沙棘特有成分齐墩果酸为对照品,采用薄层色谱法进行鉴别。结果表明,采用药典方法,阴性有干扰;采用自建方法,阴性无干扰、专属性好,确定三氯甲烷-甲醇-甲酸(40︰1︰1)为展开条件时,色谱斑点清晰、分离度好,对温度、湿度、不同厂家薄层板的耐用性较好。自建的鉴别方法操作快速、简便、准确,系统耐用性较好。To establish a TLC method for sea buckthorn identification of in a compound preparations named as Qingzhining, oleanolic acid, a characteristic component of sea buckthorn in raw material, was used as the reference substance, and TLC was used for identification. The results showed there were negative interferences when the pharmacopoeia method was used. There was no negative interferences when the self-built method was used and the specificity of the method was good. It was determined that chloroform-methanol-formic acid(40︰1︰1) as the condition and the chromatographic spots was clear with good separating degree and good durability to temperature, humidity and laminates of different manufacturers. The self-built identification method was quick, simple and accurate, and the system had good durability.

关 键 词：清旨宁胶囊 沙棘 薄层色谱法 鉴别 方法学 

分 类 号：R286.0[医药卫生—中药学]