日本虫草子实体多糖抗氧化及免疫调节作用  

Antioxidant activities and immunomodulatory effects of the polysaccharides from fruiting bodies of Cordyceps japonica

作　　者：孟菲 宗雯雯 王纯 庄晶云 江晓路[1,2] MENG Fei;ZONG Wen-Wen;WANG Chun;ZHUANG Jing-Yun;JIANG Xiao-Lu(College of Food Science and Engineering,Ocean University of China,Qingdao,Shandong 266003,China;Qingdao Marine Biomedical Research Institute,Qingdao,Shandong 266000,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]青岛海洋生物医药研究院,山东青岛266000

出　　处：《菌物学报》2020年第7期1391-1399,共9页Mycosystema

基　　金：酶法定向制备褐藻胶聚寡糖系列产品产业链构建(3007000/931769010)。

摘　　要：为明确日本虫草子实体多糖(CJPs)的抗氧化活性及免疫调节作用,本研究以子实体为原料,采用热水浸提法得多糖,通过对ABTS自由基、DPPH自由基、·OH自由基清除能力评价CJPs的体外抗氧化活性;探讨CJPs对氢化可的松诱导的免疫抑制小鼠的免疫调节作用。结果表明,CJPs对ABTS、DPPH、·OH自由基均有明显的清除效果,且存在量效关系,IC50分别为0.165、0.098、0.253mg/mL;灌胃CJPs的小鼠与模型组相比,免疫器官指数、巨噬细胞吞噬指数、血清中细胞因子水平、免疫球蛋白含量均有所提高,脾脏中乳酸脱氢酶(LDH)和酸性磷酸酶(ACP)的活力显著增强(P<0.05或P<0.01)。该研究表明CJPs是一种潜在的抗氧化剂,并能显著提高免疫低下小鼠的免疫功能,为其进一步研究和应用提供了科学依据。Antioxidant activities and immunoregulatory effects of polysaccharides from fruiting bodies of Cordyceps japonica were investigated. Cordyceps japonica polysaccharides(CJPs) were obtained by hot water extraction. The antioxidant activities of CJPs were evaluated by the scavenging ability of ABTS free radical, DPPH free radical and ·OH free radical. The immunoregulatory effects of CJPs on immunosuppressive mice injected with hydrocortisone were investigated. The results showed that CJPs exhibited a dose-dependent scavenging activity against ABTS, DPPH and ·OH radicals, and IC50 was 0.165, 0.098 and 0.253 mg/mL, respectively. Compared with model experimental group, the immune organ indexes, phagocytosis index of macrophage, cytokine levels and immunoglobulin content of mice fed with CJPs were enhanced, and LDH and ACP enzyme activities in spleen were significantly heightened(P<0.05 or P<0.01). The results suggested that CJPs were potential antioxidant and could improve the immune function of immunosuppressed mice, worthy of further research and development.

关 键 词：虫草多糖 抗氧化活性 免疫调节 

分 类 号：R285.5[医药卫生—中药学]