羟丙基壳聚糖接枝丝素肽的合成及其性能  

Synthesis and Properties of Hydroxypropyl Chitosan-Silk Fibroin Peptide

作　　者：杨晓爽 胡婉庆 张畅 樊李红 YANG Xiaoshuang;HU Wanqing;ZHANG Chang;FAN Lihong(School of Chemistry,Chemical Engineering and Life Sciences,Wuhan University of Technology,Wuhan 430070,Hubei,China)

机构地区：[1]武汉理工大学化学化工与生命科学学院,湖北武汉430070

出　　处：《武汉大学学报（理学版）》2020年第4期315-322,共8页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金(51773161)。

摘　　要：以转谷氨酰胺酶为催化剂,在水相条件下将丝素肽(SFP)接枝到羟丙基壳聚糖(HPCS)分子链上制备了一系列不同取代度的羟丙基壳聚糖-丝素肽(HPCS-SFP),采用Fourier变换红外光谱(FT-IR)表征其结构,通过单因素实验法分别探究反应温度、反应时间、反应底物质量比对HPCS-SFP取代度的影响,并考察了不同取代度HPCS-SFP的性能。实验结果表明,随着取代度的增加,HPCS-SFP清除DPPH(1,1-二苯基-2-三硝基苯肼)自由基和羟基自由基的能力增强,说明丝素肽的引入提高了抗氧化性能;吸湿保湿性能也随着取代度的增加而提高;同时,HPCS-SFP还具有良好的细胞相容性。In this paper, silk fibroin peptide(SFP) was grafted onto hydroxypropyl chitosan(HPCS) molecular chain under the catalysis of transglutaminase in aqueous conditions to obtain a series of hydroxypropyl chitosan-silk fibroin peptide(HPCS-SFP)with different degree of substitution(DS). The structure of HPCS-SFP was characterized by Fourier transform infrared(FT-IR) spectrometer. The effects of reaction temperature, reaction time and mass ratio on DS of HPCS-SFP were investigated by single-factor experiments. The antioxidant property, moisture absorption property, moisture retention property and biocompatibility were explored. The results show that with the increase of DS, the ability of HPCS-SFP to scavenge DPPH radicals and hydroxyl radicals increases, indicating that the addition of silk fibroin peptides improvs its antioxidant performance. At the same time, it is found that the moisture absorption and retention properties of HPCS-SFP are enhanced with the increase of DS. In addition, HPCS-SFP has good biocompatibility.

关 键 词：羟丙基壳聚糖 丝素肽 转谷氨酰胺酶 抗氧化性 

分 类 号：O636.1[理学—高分子化学]