甘草黄酮的提取工艺优化、含量测定及体外抗氧化活性研究  

Extraction Process Optimization,Content Determination,and in vitro Antioxidant Activity of Licoflavone

作　　者：张光辉[1] 张拴[1] 靳如意 孟庆华[1] ZHANG Guanghui;ZHANG Shuan;JIN Ruyi;MENG Qinghua(College of Pharmacy,Shaanxi University of Chinese Medicine,Xi′an 712046,China)

机构地区：[1]陕西中医药大学药学院,陕西西安712046

出　　处：《化学与生物工程》2020年第8期17-21,共5页Chemistry & Bioengineering

基　　金：陕西省科技攻关计划项目(2016SF-102);陕西省教委基金项目(19JK0235)。

摘　　要：以95%乙醇为提取溶剂提取甘草黄酮,以提取率为评价指标,采用响应面法优化提取工艺;以芦丁为对照品,采用亚硝酸钠-硝酸铝-氢氧化钠比色法测定不同产地甘草黄酮含量;通过DPPH自由基清除反应,比较不同产地甘草黄酮的体外抗氧化活性。结果表明,醇提法提取甘草黄酮的最优工艺条件为:提取温度86.7℃、提取时间4.0 h、液料比80.0∶1.0(mL∶g);在此工艺条件下,甘肃、新疆、内蒙古的甘草黄酮含量分别为18.22 mg·g^-1、17.75 mg·g^-1、16.82 mg·g^-1,其对DPPH自由基的清除率分别为63.82%、62.74%、51.41%;3个产地的甘草黄酮的含量及体外抗氧化活性大小顺序均为:甘肃>新疆>内蒙古。该结论可作为甘草原产地鉴别及质量评价的参考依据之一,为甘草药材的开发利用提供帮助。Using 95%ethanol as an extraction solvent and extraction rate as an evaluation index,we optimized the extraction process of licoflavone from different producing areas by response surface methodologies.Moreover,using rutin as a reference substance,we determined the content of licoflavone from different producing areas by sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method.Furthermore,we compared in vitro antioxidant activity of licoflavone from different producing areas by scavenging DPPH free radicals.The results show that the optimum ethanol extraction conditions of licoflavone are determined as follows:the extraction temperature of 86.7℃,the extraction time of 4.0 h,and the liquid-solid ratio of 80.0∶1.0(mL∶g).Under above conditions,the contents of licoflavone in Gansu,Xinjiang,and Inner Mongolia are 18.22 mg·g^-1,17.75 mg·g^-1,and 16.82 mg·g^-1,respectively,and the scavenging rates of DPPH free radicals are 63.82%,62.74%,and 51.41%,respectively.The order of the licoflavone content and the in vitro antioxidant activity of three producing areas is Gansu>Xinjiang>Inner Mongolia.This conclusion can be used as one of the references for origin identification and quality evaluation of licorice,and can provide help for the development and utilization of licorice.

关 键 词：甘草黄酮 响应面法 工艺优化 含量测定 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学] R932[医药卫生—中医学]