微生物来源淀粉分支酶异源高效表达策略的研究进展  

Recent advances in high-efficiency heterologous expression strategies of microbial-derived starch branching enzyme

作　　者：管媛媛 杨婷 葛雨嘉 黄静[1] GUAN Yuanyuan;YANG Ting;GE Yujia;HUANG Jing(School of Life Sciences,East China Normal University,Shanghai 200241,China)

机构地区：[1]华东师范大学生命科学学院,上海200241

出　　处：《食品与发酵工业》2020年第16期276-282,共7页Food and Fermentation Industries

基　　金：大学生创新创业训练计划项目(2019PY-367)。

摘　　要：淀粉分支酶(starch-branching enzyme,SBE,EC 2.4.1.18)是一种参与淀粉生物合成的糖基转移酶,能切断淀粉分子中的α-1,4-糖苷键,由此形成的非还原性低聚糖通过α-1,6-糖苷键连接至受体链上,形成α-1,6分支位点,经过"切割-转移-连接"多次反应获得一种新型生物改性淀粉——高支化淀粉。微生物来源的SBE具有产量高、稳定性好、易于异源表达等优势,是生产大量SBE的研究热点。在众多酶表达宿主中,大肠杆菌和枯草芽孢杆菌是SBE异源表达的最常见宿主菌。该文着重阐述了近年来有关实现微生物来源的SBE在以上2种宿主内异源高效表达策略的研究进展,为深入探究SBE作用机理及改性淀粉的工业化应用提供了重要参考。Starch-branching enzyme (SBE,EC 2. 4. 1. 18) is a glycosyltransferase involved in starch biosynthesis. It can cut the α-1,4-glycosidic bond in starch molecules and form non-reducing oligosaccharide connecting to the receptor chain through α-1,6-glycosidic bond to form α-1,6 branching sites. Through these "cutting-transfer-linking"processes,a new type of biologically modified starch named highly branched starch can be obtained. Microbial-derived starch branching enzymes have the advantages of high yield and better stability as well. It can be easily expressed in heterologous hosts.As a consequence,mass production of SBE has become a research hotspot in recent years. Among many hosts for enzyme expression,Escherichia coli and Bacillus subtilis are the most common host strains for heterologous expression of SBE. This paper focused on recent advances in strategies for highefficiency heterologous expression of microbial-derived SBEs in these two hosts,providing important references for further investigation of the mechanism of SBE function and the industrialized application of modified starch.

关 键 词：淀粉分支酶 异源表达 改性淀粉 大肠杆菌 枯草芽孢杆菌 

分 类 号：TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]