小叶榕叶片挥发物抑菌活性的研究  

Study on the Antimicrobial Activity of Volatile Compounds from Ficus microcarpa Leaves

作　　者：李佛生[1] 汪红[1] 牛壮 赵天德 肖渝霖 吴宛峰 郑冬超 LI Fosheng;WANG Hong;NIU Zhuang;ZHAO Tiande;XIAO Yulin;WU Wanfeng;ZHENG Dongchao(College of Life Sciences,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学生命科学学院,成都610065

出　　处：《实验科学与技术》2020年第4期39-43,共5页Experiment Science and Technology

基　　金：四川省能源微生物资源共享平台项目(19PTDJ0029);教育部基础学科拔尖学生培养计划(20180209)。

摘　　要：为了研究小叶榕叶片挥发物的抑菌活性,该文以小叶榕叶片挥发物吸收液和叶片研磨物为材料,通过平板计数法和涂布密封法检测其对指示菌种金黄色葡萄球菌的抑菌效果;并通过固相微萃取结合气相色谱−质谱联用法检测叶片研磨物中的有效抑菌成分。结果显示:小叶榕叶片挥发物吸收液和叶片研磨物均对金黄色葡萄球菌具有明显的抑菌作用,且抑菌效应与叶片生物量呈正相关关系,新叶的抑菌效果高于老叶;叶片中所含的有效抑菌成分主要是己烯醛和己烯醇类,两者比例的不同可能是造成新叶与老叶抑菌效应差异的原因。本研究证实了小叶榕叶片挥发物的抑菌活性,为小叶榕抑菌效用的开发和推广提供了理论依据。In order to study the antimicrobial activity of volatiles from Ficus microcarpa leaves,the absorption solution of volatiles from Ficus microcarpa leaves and abrasives of leaves were used as materials.The antimicrobial activity of volatiles from Ficus microcarpa leaves against Staphylococcus aureus was tested by plate count method and coating sealing method.The effective antimicrobial components in leaf abrasives were detected by solid phase microextraction combined with gas chromatography-mass spectrometry.The results showed that both volatile absorbent and abrasive of Ficus microcarpa leaves had obvious bacteriostatic effect on Staphylococcus aureus,and the bacteriostatic effect was positively correlated with the biomass of leaves.The bacteriostatic effect of new leaves was higher than that of old leaves.The effective bacteriostatic components in leaves were mainly hexenal and hexenol,and the different proportion of these two organic substances might cause the different bacteriostatic effect between new and old leaves.This study has confirmed the bacteriostatic activity of volatiles from Ficus microcarpa leaves,and provided theoretical basis for the development and promotion of bacteriostatic effect of Ficus microcarpa.

关 键 词：小叶榕 叶片挥发物 平板计数法 涂布密封法 金黄色葡萄球菌 抑菌活性 

分 类 号：G642.423[文化科学—高等教育学] Q939.9[文化科学—教育学]