Glycogen synthase kinase-3β inhibitor SB216763 promotes DNA repair in ischemic retinal neurons  

出　　处：《Neural Regeneration Research》2021年第2期394-400,共7页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China,Nos.81670848(to JZhuang)and 81900850(to YY)。

摘　　要：Glycogen synthase kinase-3β(GSK-3β) has been shown to attenuate DNA damage in nerve cells, thereby enhancing neuronal survival under pathological conditions;however, the underlying mechanism remains unclear. An in vitro serum-starvation retinal neuron model and in vivo ischemia/reperfusion retina injury rat model were established and treated with SB216763, a GSK-3β inhibitor. SB21673 decreased the formation of γ-H2 A histone family member X foci and enhanced the viability of ischemic retinal neurons. In addition, SB216763 upregulated expression of phosphorylated-CREB1, a ligase IV transcription factor, and significantly increased the transcriptional activity of ligase IV in ischemic retinal neurons. These results were confirmed in rat retinas following ischemia/reperfusion injury. Furthermore, we found that unlike lithium chlorine(a well-known direct inhibitor of GSK-3β), SB216763 inhibited GSK-3β activity by suppressing its phosphorylation. Taken together, our results suggest that GSK-3β inhibition enhances repair of DNA double-strand breaks by upregulating ligase IV expression in ischemic retinal neurons. This study was approved by the Institutional Animal Care and Use Committee of Zhongshan Ophthalmic Center on February 18, 2018.

关 键 词：factor GSK-3Β in vitro injury ligase IV NEUROPROTECTION optic nerve PATHWAYS protein repair 

分 类 号：R774.1[医药卫生—眼科]