淫羊藿朝藿定F的微波-超声波协同提取及其对α-葡萄糖苷酶和α-淀粉酶活力的影响  

Microwave-ultrasound assisted extraction of epimedin F from Epimedii Folium and its effects onα-glucosidase andα-amylase

作　　者：安叶娟 周雪雪 王艺 杨咪 张华峰[1] AN Yejuan;ZHOU Xuexue;WANG Yi;YANG Mi;ZHANG Huafeng(School of Food Engineering and Nutritional Science,Shaanxi Normal University,National Engineering Laboratory for Resources Development of Endangered Crude Drugs in Northwest China,International Joint Research Center of Shaanxi Province for Food and Health Sciences,Xi′an 710119,Shaanxi,China)

机构地区：[1]陕西师范大学食品工程与营养科学学院,西北濒危药材资源开发国家工程实验室,中俄食品与健康科学国际联合研究中心,陕西西安710119

出　　处：《陕西师范大学学报（自然科学版）》2020年第5期26-33,共8页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：陕西省科学技术研究发展计划(2017GHJD-006);中央高校基本科研业务费专项资金(GK2016CSZ008,GK201703076)。

摘　　要：采用响应面实验方法优化药食两用植物淫羊藿中朝藿定F的微波-超声波协同提取工艺,利用扫描电子显微镜和透射电子显微镜技术探讨提取机理,并研究朝藿定F对α-葡萄糖苷酶和α-淀粉酶活力的抑制作用。朝藿定F的最优微波-超声波协同提取工艺为:乙醇体积分数70%,提取时间20 min,液固比20∶1(mL∶g),微波功率200 W。微波-超声波协同提取法的提取率比传统的微波提取法高13%,比超声波提取法高23%,其机理可能是微波-超声波协同处理促进了淫羊藿组织、细胞和部分细胞器的破损,加速了朝藿定F的溶出。朝藿定F对α-葡萄糖苷酶具有一定的抑制作用,其半抑制浓度IC 50为66.89μg/mL。A microwave-ultrasound assisted extraction procedure was optimized to isolate epimedin F from Epimedii Folium using response surface test.The extraction mechanism was discussed by scanning and transmission electron micrographs.Inhibitory activities of epimedin F againstα-glucosidase andα-amylase were analyzed.The optimal extraction conditions were as follows:ethanol concentration,70%;extraction duration,20 min;ratio of liquor to solid,20∶1(mL∶g);microwave power,200 W.The extraction yield of microwave-ultrasound assisted extraction was 13%higher than that of the microwave assisted extraction and 23%higher than that of the ultrasound assisted extraction.This could be elucidated by the disruptions of tissues,cells and organelles,which facilitated the dissolution of epimedin F.Epimedin F exhibited inhibitory effect onα-glucosidase with the IC 50 value of 66.89μg/mL.This study provides a reference for the further development of Epimedii Folium resources and its application in auxiliary hypoglycemic health food.

关 键 词：淫羊藿 朝藿定F 微波-超声波协同提取 超微结构 Α-葡萄糖苷酶 Α-淀粉酶 

分 类 号：O614[理学—无机化学]