超高效液相色谱-三重四级杆质谱法测定猪肉中氯霉素残留量的不确定度评定  

Uncertainty evaluation for the determination of chloramphenicol residue in pork by high performance liquid chromatography-tandem mass spectrometry

作　　者：温海滨 熊含鸿 梁旭霞 雷毅 WEN Hai-Bin;XIONG Han-Hong;LIANG Xu-Xia;LEI Yi(Guangdong Institute of Food Inspection,Guangzhou 510435,China)

机构地区：[1]广东省食品检验所,广州510435

出　　处：《食品安全质量检测学报》2020年第16期5448-5454,共7页Journal of Food Safety and Quality

基　　金：广东省科技计划项目(2019B020208008);广东省食品药品监督管理局科技创新项目(2018TDB07,2018TDB09)。

摘　　要：目的采用超高效液相色谱-三重四级杆质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)测定猪肉中的氯霉素残留量,并进行不确定度评定。方法依据JJF1059.1-2012《测量不确定度评定与表示》,结合数学模型和实验过程,分析测定结果的主要测量不确定度来源,评定各标准不确定度的分量。结果不确定度主要来源于标准溶液配制和标准曲线拟合。当猪肉中氯霉素残留量测定结果为1.07μg/kg时,其扩展不确定度为0.12μg/kg,结果表示为(1.07±0.12)μg/kg (k=2)。结论该评定方法适用于超高效液相色谱-串联质谱法测定猪肉中氯霉素残留量的不确定度分析,可以为科学评价药物残留测量结果的准确性提供依据。Objective To evaluate the uncertainty of chloramphenicol residue in pork by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Methods According to JJF 1059.1-2012 Evaluation and expression of uncertainty in measurement, combined with mathematical model and experimental process, the main sources of uncertainty resulted from the determination process were analyzed, and the components of each standard uncertainties were evaluated. Results The uncertainties of the method were mainly derived from standard solution preparation and standard curve calibration. When the chloramphenicol residue in pork was 1.07 μg/kg, the extended uncertainty was 0.12 μg/kg, and the result was expressed as(1.07±0.12) μg/kg(k=2). Conclusion The evaluation method is suitable for the uncertainty analysis of chloramphenicol in pork determined by UPLC-MS/MS, which provides a basis for scientific evaluation of the accuracy of drug residue measurement results.

关 键 词：超高效液相色谱-串联质谱法 猪肉 氯霉素 不确定度 

分 类 号：O657.63[理学—分析化学] TS251.51[理学—化学]