三相分离法提取胡椒油树脂的工艺研究  

Processing Parameters of Extracting Pepper Oleoresin by Three Phase Partitioning

作　　者：陈星星 宋丽 谷风林[2,4,5] 吴桂苹 陶锐[6] CHEN Xingxing;SONG Li;GU Fenglin;WU Guiping;TAO Rui(College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China;Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wanning,Hainan 571533,China;School of Liquor and Food Engineering/Guizhou Province Key Laboratory of Agricultural and Animal Products Processing and Storage,Guizhou University,Guiyang,Guizhou 550025,China;National Center of Important Tropical Crops Engineering and Technology Research,Wanning,Hainan 571533,China;Hainan Provincial Engineering Research Center of Tropical Spice and Beverage Crops,Wanning,Hainan 571533,China;Hainan Haiken Pepper Industry Co.,Ltd.,Haikou,Hainan 571100,China)

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070 [2]中国热带农业科学院香料饮料研究所,海南万宁571533 [3]贵州大学酿酒与食品工程学院/贵州省农畜产品贮藏与加工重点实验室,贵州贵阳550025 [4]国家重要热带作物工程技术研究中心,海南万宁571533 [5]海南省热带香料饮料作物工程技术研究中心,海南万宁571533 [6]海南海垦胡椒产业股份有限公司,海南海口571100

出　　处：《热带作物学报》2020年第8期1674-1682,共9页Chinese Journal of Tropical Crops

基　　金：海南省重大科技计划项目(No.zdkj201814)。

摘　　要：本文以黑胡椒粉为原料,采用三相分离法(TPP)提取胡椒油树脂,研究了胡椒粉添加量、硫酸铵质量浓度、提取液与叔丁醇体积比、pH、温度对胡椒油树脂得率及胡椒碱含量的影响,并通过响应面试验优化了胡椒油树脂的提取工艺。结果表明:最佳提取条件为胡椒粉添加量5%、硫酸铵质量浓度10%、提取液与叔丁醇的体积比1∶0.5、pH 4、温度20℃,在此条件下,黑胡椒油树脂得率为12.90%,黑胡椒油树脂中胡椒碱含量为30.46%,验证试验与模型预测值基本相符。研究结果为工业化提取胡椒油树脂提供参考。In this study,three phase partitioning(TPP)was employed to extract the pepper oleoresin from black pepper powder.The effects of the addition amount of pepper powder,ammonium sulphate concentration,ratio of crude extract to t-butanol,pH and temperature on the yield of pepper oleoresin and piperine content were studied.In addition,the extraction process of pepper oleoresin was optimized by a response surface test.The optimal condition obtained was as follow:the addition amount of pepper powder 5%,ammonium sulphate concentration 10%,ratio of crude extract to t-butanol 1∶0.5,pH 4 and temperature 20℃.Under the optimal conditions,the yield of black pepper oleoresin and piperine content was 12.90%and 30.46%,respectively.The results provide a reference for the industrial extraction of pepper oleoresin.

关 键 词：胡椒油树脂 三相分离法 胡椒碱 提取 

分 类 号：S573[农业科学—作物学] TS205[轻工技术与工程—食品科学]