Triterpenoid content and expression of triterpenoid biosynthetic genes in birch(Betula platyphylla Suk)treated with 5-azacytidine  

作　　者：Fansuo Zeng Xiaoyi Li Rui Qie Leilei Li Minghao Ma Yaguang Zhan 

机构地区：[1]State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,People’s Republic of China [2]College of Life Science,Northeast Forestry University,Harbin 150040,People’s Republic of China [3]First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,People’s Republic of China

出　　处：《Journal of Forestry Research》2020年第5期1843-1850,共8页林业研究（英文版）

基　　金：The work was supported by The Fundamental Research Funds for the Central Universities(2572017EA05);the National Natural Science Foundation of China(31870588).

摘　　要：DNA methylation is widespread in plants and associated with plant development and defense mechanisms.However,the relationship between DNA methylation and plant secondary metabolism has rarely been reported.Here,when birch suspension cells were treated with 5-azacytidine(5-azaC),which blocks DNA methylation,triterpenoid accumulation was significantly promoted and antioxidant and defense enzymatic activity changed.For studying triterpenoid accumulation,0.1 mM azaC was optimal.A qRT-PCR assay revealed increased expression of genes encoding key triterpenoid biosynthetic enzymes.Evaluation of methylation polymorphisms at CCGG sites showed that the methylation level was lower in cells treated with 5-azaC.These results demonstrated that 5-azaC treatment led to an increase in the production of triterpenoids in cell cultures through a mechanism that involved in DNA methylation,which resulted in the induction of genes encoding the key enzymes.The study provides evidence of a relationship between DNA methylation and regulation of secondary metabolism.

关 键 词：5-AZACYTIDINE METHYLATION Suspension cells Triterpenoids biosynthesis 

分 类 号：S792.15[农业科学—林木遗传育种]