基因AL360181.1调节结直肠癌进展及预后的生物信息学分析  

Bioinformatics analysis of AL360181.1 regulating the progression and prognosis of colorectal cancer

作　　者：史维俊 李欣灿 温贺新 吴华彰[3] 刘牧林[1] SHI Weijun;LI Xincan;WEN Hexin;WU Huazhang;LIU Mulin(Department of Gastroenterology,The First Affiliated Hospital of Bengbu Medical College;Department of General Medicine,The Second Affiliated Hospital of Bengbu Medical College;Department of Biomedicine,Bengbu Medical College,Bengbu 233003,China)

机构地区：[1]蚌埠医学院第一附属医院胃肠外科 [2]蚌埠医学院第二附属医院全科医学科 [3]蚌埠医学院生物医学系,安徽蚌埠233003

出　　处：《西安交通大学学报（医学版）》2020年第5期731-737,共7页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：安徽省自然科学基金资助项目(No.1908085MH257);安徽省高校自然科学研究项目(No.gxyq2018035);蚌埠医学院科研创新团队(No.BYKC201909);蚌埠医学院转化医学重点专项(No.BYTM2019008);蚌埠医学院研究生科研创新计划项目(No.Byycx1902)。

摘　　要：目的通过生物信息学方法分析基因AL360181.1在结直肠癌进展及预后中的作用,并进一步预测可能的分子机制。方法从TCGA数据库下载结直肠癌基因表达数据及临床数据,应用Perl及R软件对数据整理后筛选出差异表达的基因,进一步对差异基因批量化行生存、独立预后及临床病理相关性分析,选取相关性最强的差异基因作为目的基因;分析目的基因的表达与结直肠癌不同临床病理特征的相关性及对患者预后的影响;采用R软件对不同的临床病理特征行独立预后分析;分析目的基因以及不同临床病理与患者生存预后的关系;应用GSEA对目的基因行KEGG通路富集分析并利用R软件分析目的基因与潜在靶基因的相关性,预测目的基因调控结直肠癌进展及预后可能的机制。结果基因AL360181.1在结直肠癌组织中显著高表达(P<0.01);AL360181.1与结直肠癌的分级、TNM分期具有相关性(P<0.05);基因AL360181.1、年龄、肿瘤分级、TNM分期与患者预后相关(P<0.05),且基因AL360181.1及年龄可作为评价患者预后的独立风险因子;基因AL360181.1的表达水平、患者年龄、分级及TNM分期与患者的生存状态相关(P<0.05);GSEA富集分析显示基因AL360181.1在mTOR信号通路中被富集;基因AL360181.1与潜在靶基因的相关性分析显示,AL360181.1与mTOR、AKT、KLKB1、RICTOR、TSC1、TSC2具有相关性(|R|>0.1、P<0.05)。结论基因AL360181.1可通过AKT/mTOR信号通路调节结直肠癌的进展。Objective To analyze the role of gene AL360181.1 in the progression and prognosis of colorectal cancer by bioinformatics method to further predict possible molecular mechanisms.Methods The colorectal cancer gene expression data and clinical data were downloaded from the TCGA database.The data were analyzed by Perl and R software,and the differentially expressed genes were screened to further analyze the survival,independent prognosis and clinicopathological correlation of differential gene batches.The most relevant differential gene was selected as the target gene;the correlation between the expression of target gene and different clinicopathological features of colorectal cancer and its prognosis were analyzed.The independent prognosis analysis was performed by using R software for different clinicopathological features.The relationship between the target gene and different clinical pathology and patient survival prognosis was analyzed. Finally, GSEA was used to analyze the target gene KEGG pathway and the correlation between target gene and potential target gene was analyzed with R software. We predicted the probable mechanisms of the target gene in regulating the progression and prognosis of colorectal cancer. Results AL360181.1 was highly expressed in colorectal cancer tissues ( P <0.01). AL360181.1 was correlated with the grade and TNM stage of colorectal cancer ( P <0.05). Gene AL360181.1, age, tumor grade and TNM staging were associated with the prognosis ( P <0.05);gene AL360181.1 and age were used as independent risk factors to evaluate patient prognosis. The expression level of gene AL360181.1, patient age, grade and TNM staging had correlation with the patients survival status ( P <0.05). GSEA enrichment analysis showed that gene AL360181.1 was enriched in mTOR signaling pathway;the correlation analysis of gene AL360181.1 with potential target genes showed that AL360181.1 had correlations with mTOR, AKT, KLKB1, RICTOR, TSC1 and TSC2 (| R |>0.1, P <0.05). Conclusion Gene AL360181.1 regulates the progres

关 键 词：AL360181.1 结直肠癌 MTOR 进展及预后 生物信息学分析 

分 类 号：R363[医药卫生—病理学]