HPLC-ABTS^+·在线检测快速评价茶叶总抗氧化能力  

Rapid Evaluation for the Total Antioxidant Capacity of Tea by HPLC-ABTS^+ · On-Line Detection

作　　者：王丹[1] 时志春 李军[1] 王金兰[1] 张树军[1] 赵明[1] WANG Dan;SHI Zhichun;LI Jun;WANG Jinlan;ZHANG Shujun;ZHAO Ming(Institute of Chemistry and Chemical Engineering,Qiqihar University,Qiqihar 161006)

机构地区：[1]齐齐哈尔大学化学与化学工程学院,齐齐哈尔161006

出　　处：《食品工业》2020年第8期280-283,共4页The Food Industry

基　　金：2018年黑龙江省省属高等学校基本科研业务费项目(YSTSXK201808);2017年黑龙江省教育厅基本业务专项粮头食尾项目(LTSW201734)。

摘　　要：利用HPLC-ABTS^+·在线检测系统分别对花茶、西湖龙井、信阳毛尖、铁观音的总抗氧化活性进行分析,并对在线抗氧化活性检测系统的分析结果进行DPPH离线方法验证,两种方法测定结果基本一致。结果表明,4种茶叶的抗氧化活性明显不同,花茶的抗氧化活性最强,总的抗氧化活性排序依次为花茶>西湖龙井>信阳毛尖>铁观音。在线检测系统适用于茶叶样品总抗氧化能力的快速测定。In this study,the total antioxidant activities of Scented tea,Longjing,Maojian and Tieguanyin were evaluated by online high-performance liquid chromatography HPLC-ABTS+·screening system,and DPPH offline method was used to validate the analysis results of online antioxidant activity detection system.The result of the two detection methods were alike,which indicated that the antioxidant activities of four kinds of tea were obviously different.Scented tea had the highest antioxidant activity in the declining order of Scented tea>Longjing>Maojian>Tieguanyin.The on-line detection system was suitable for rapid determination of total antioxidant capacity of tea samples.

关 键 词：HPLC-ABTS^+· 在线检测 总抗氧化活性 茶叶 

分 类 号：O657.72[理学—分析化学] TS272.7[理学—化学]