食管鳞癌根治术患者组织中CD8^+/FOXP3^+比值及PD-L1表达与预后的相关性  

The Correlation Between CD8^+/FOXP3^+Ratio PD-L1 Expression in Tissues and Prognosis in Patients Undergoing Radical Operation of Esophageal Squamous Carcinoma

作　　者：张成伟 唱宽 毛志刚 舒建 ZHANG Chengwei;CHANG Kuan;MAO Zhigang(Air Force Hospital, Northern Theater Command of the Chinese People's Liberation Army, Liaoning Shenyang 110042, China)

机构地区：[1]中国人民解放军北部战区空军医院心胸外科,辽宁沈阳110042

出　　处：《河北医学》2020年第9期1441-1445,共5页Hebei Medicine

基　　金：辽宁省卫生厅科研基金项目,(编号:2017D05215)。

摘　　要：目的:探讨食管鳞癌(ESCC)根治术患者组织中CD8^+/FOXP3^+比值及程序性死亡分子配体1(PD-L1)表达与预后的相关性。方法:将2014年1月至2019年1月我院行ESCC根治术患者的存档蜡块和癌旁正常组织(距病变部位>5cm处,且证实为无癌浸润)石蜡标本各110例作为研究对象。采用免疫组化法检测ESCC组织中CD8^+、FOXP3+、PD-L1的表达与患者病理参数、预后的关系。结果:CD8^+/FOXP3^+在ESCC组织中的阳性表达率低于癌旁组织,PD-L1在ESCC组织中的阳性表达率高于癌旁组织(P<0.05)。单因素和多因素分析显示,TNM T3期分期、合并淋巴结转移、PD-L1阳性表达、CD8^+/FOXP3^+阴性表达是影响ESCC患者预后的独立危险因素(P<0.05)。结论:CD8^+/FOXP3^+阳性表达、PD-L1阴性表达、TNM分期为T3期、有淋巴结转移是影响ESCC根治术后患者预后的独立危险因素。Objective To explore the correlation between CD8^+/FOXP3^+,expression of programmed death ligand 1(PD-L1)in tissues and prognosis of patients undergoing radical operation of esophageal squamous carcinoma(ESCC).Methods:During the period from January 2014 to January 2019,110 archived wax specimens and 110 wax specimens of normal tissues adjacent to the cancer(longer than 5 cm away from lesion,confirmed as cancer-free infiltration)from patients who underwent radical operation of ESCC in the hospital were enrolled as study objects.The expression of CD8^+,FOXP3+and PD-L1 in ESCC tissues was detected by immunohistochemistry,and the relationship between pathological parameters and prognosis was analyzed.Results:The positive expression rate of CD8^+/FOXP3^+in ESCC tissues was lower than that in adjacent tissues,while the positive expression rate of PD-L1 was higher than adjacent tissues(P<0.05).Univariate and multivariate analysis showed that TNM stage T3,lymph node metastasis,positive expression of PD-L1 and negative expression of CD8^+/FOXP3^+were independent risk factors affecting prognosis of ESCC patients(P<0.05).Conclusion:Positive expression of CD8^+/FOXP3^+,negative expression of PD-L1,TNM stage T3 and lymph node metastasis are independent risk factors affecting prognosis of patients after radical operation of ESCC.

关 键 词：食管鳞癌 根治术 CD8^+/FOXP3^+比值 PD-L1 

分 类 号：R73[医药卫生—肿瘤]