日本沼虾原肌球蛋白线性表位预测研究  

Prediction of Linear Epitope of Tropomyosin in Macrobrachium nipponense

作　　者：华希玮 谢彦海 陈红兵[1,4] HUA Xi-wei;XIE Yan-hai;CHEN Hong-bing(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;College of Food Science and Technology,Nanchang University,Nanchang 330047,China;Science Center of Laboratory Animal,Nanchang University,Nanchang 330006,China;Sino-German Joint Research Institute,Nanchang University,Nanchang 330047,China)

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学食品学院,江西南昌330047 [3]南昌大学实验动物科学中心,江西南昌330006 [4]南昌大学中德联合研究院,江西南昌330047

出　　处：《食品工业科技》2020年第17期84-90,97,共8页Science and Technology of Food Industry

基　　金：国家高技术研究发展计划(863计划)项目(2013AA102205);南昌大学食品科学与技术国家重点实验室开放基金课题(SKLF-KF-201806);江西省博士后基金项目(2016KY23)。

摘　　要：本文旨在预测日本沼虾原肌球蛋白的线性表位,为相关食物过敏的识别与检测提供依据,同时为基于抗原表位的致敏性消减提供靶标。采用DNAStar软件、SOPMA、BepiPred 1.0 Server及ABCpred在线网站预测日本沼虾原肌球蛋白的B细胞线性表位,应用SYFPEITHI、NetMHCII 2.3 Server与NetMHCIIpan 3.2 Server预测T细胞表位。综合分析以上预测结果,表明日本沼虾原肌球蛋白可能的B细胞线性表位有21RADTLEQQNKEANN^34、37EKTEEEIRTTQKKMQQ^52、71LEEKEKA^77、99LERSEERLN^107、119AADESER^125、134SLSDEER^140、158ADRKYDE^164、177ERAEERAETG^186、210SEEKANQREEAYKE^223、262NEKEKYK^268,可能的T细胞表位有82EGEVAALNRRIQLL^95、105RLNTATTKLAEAS^117、165VARKLAMVEADLE^177、195EELRVVGNNLKSLE^208、222KEQIKTLTNKLKAA^235。该结果可为继续深入开展日本沼虾过敏原基础性研究提供更精准的靶标。The linear epitopes of tropomyosin in M.nipponense were predicted,which provided the basis for the identification and detection of related food allergies and the targets for the reduction of allergenicity based on the epitope.In this study,the B-cell linearepitopes of tropomyosin in M.nipponense were predicted by DNAStar and online network servers including SOPMA,BepiPred 1.0 Server and ABCpred.The T-cell epitopes were predicted by SYFPEITHI,NetMHCII 2.3 Server and NetMHCIIpan 3.2 Server.Based on the above comprehensive analysis,the probable B-cell linear epitopes might contain 21RADTLEQQNKEANN^34,37EKTEEEIRTTQKKMQQ^52,71LEEKEKA^77,99LERSEERLN^107,119AADESER^125,134SLSDEER^140,158ADRKYDE^164,177ERAEERAETG^186,210SEEKANQREEAYKE^223,262NEKEKYK^268 and the possible T-cell epitopes were 82EGEVAALNRRIQLL^95,105RLNTATTKLAEAS^117,165VARKLAMVEADLE^177,195EELRVVGNNLKSLE^208,222KEQIKTLTNKLKAA^235.This research can provide a more accurate target for further study on M.nipponense allergen.

关 键 词：日本沼虾 原肌球蛋白 B细胞表位 T细胞表位 预测 

分 类 号：TS251.95[轻工技术与工程—农产品加工及贮藏工程]