添加Ni^2+ 强化 Klebsiella sp.发酵棉秆水解糖液产氢性能  

Reinforcement of hydrogen production capability of Klebsiella sp. fromfermentation of cotton stalk hydrolysate via addition of Ni^2+

作　　者：尤少林 李艳宾 蒋卉[2] 张琴 YOU Shao-lin;LI Yan-bin;JIANG Hui;ZHANG Qin(College of Biological and Chemical Engineering,Anhui Polytechnic University,Wuhu 241000,Anhui Province,China;College of Life Science,Tarim University,Alaer 843300,Xinjiang,China)

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000 [2]塔里木大学生命科学学院,新疆阿拉尔843300

出　　处：《化学工程》2020年第6期1-5,56,共6页Chemical Engineering(China)

基　　金：国家自然科学基金资助项目(21406150);安徽工程大学引进人才科研启动基金(2018YQQ029);安徽省重点研究与开发项目(201904a07020003)。

摘　　要：将不同质量浓度Ni^2+添加至棉秆水解糖液发酵产氢系统,研究了Ni^2+添加对Klebsiella sp.发酵棉秆水解糖液合成生物氢过程的影响。结果表明:在Ni^2+添加质量浓度为20 mg/L处理下,Klebsiella sp.能获得最高的累积产氢量,其发酵120 h的累积产氢量较之对照组提高约24%。该添加质量浓度下,菌株通过Gompertz方程对累积产氢量拟合的动力学参数——产氢潜力P和最大产氢速率R m值最高,分别可达2957.65 mL/L和22.10 mL/(L·h),延滞期λ最短,仅为2.32 h。该添加质量浓度下,葡萄糖和木糖消耗率、ΔOD 600均值最高,较之对照组分别提高12.5%,5%和14.7%,说明适当质量浓度Ni^2+添加至Klebsiella sp.发酵棉秆水解糖液产氢系统有利于生物氢的合成、还原糖的消耗及菌体的生长。本研究结果为Klebsiella sp.更高效产氢和棉秆水解液更有效转化利用提供了理论基础和技术参考。Different mass concentrations of Ni^2+were added to the fermentative hydrogen production system of cotton stalk hydrolysate,the effects of Ni^2+addition on the biohydrogen synthesis process from fermentation of cotton stalk hydrolysate by Klebsiella sp.were studied.The results show that in the treatment with addition of 20 mg/L Ni^2+,the highest cumulative hydrogen production is obtained by Klebsiella sp.,and its cumulative hydrogen production during 120 h fermentation increases about 24%comparing with the control treatment.Moreover,under this mass concentration,the dynamical parameters——hydrogen production potential P and maximum hydrogen production rate R m are the highest,which reach 2957.65 mL/L and 22.10 mL/(L·h),respectively,and the lag phaseλis the shortest,only 2.32 h.In addition,the optimum glucose and xylose utilization andΔOD 600 are also obtained at this mass concentration,which are increased about 12.5%,5%and 14.7%comparing with the control treatment.This illustrates that a certain mass concentration of Ni^2+added to the hydrogen production system from fermentation of cotton stalk hydrolysate by Klebsiella sp.might be beneficial to the biohydrogen synthesis,and reducing sugar consumption and bacterial growth.The results provides theoretical basis and technical reference for more efficient hydrogen production of Klebsiella sp.and more effective conversion and utilization of cotton stalk hydrolysate.

关 键 词：Ni^2+添加 Klebsiella sp. 棉秆水解糖液 产氢 发酵 

分 类 号：TQ929.1[轻工技术与工程—发酵工程]