激素和抑制剂对乳腺组织形态和乳蛋白合成的影响  

Effects of hormones and inhibitors on the morphology and milk protein synthesis in mammary gland tissue

作　　者：田青[1] 王海平[1] 黄和升[1] 梁天林 李萌[1] TIAN Qing;WANG Haiping;HUANG Hesheng;LIANG Tianlin;LI Meng(Jiangsu Food&Pharmaceutical Science College,Huaian,Jiangsu Province 223003,China;Jiangsu Dachuan Biotechnology Co.LTD,Huaian,Jiangsu Province 223003,China)

机构地区：[1]江苏食品药品职业技术学院,江苏淮安223003 [2]江苏大川生物科技有限公司,江苏淮安223003

出　　处：《中国饲料》2020年第17期58-62,66,共6页China Feed

基　　金：江苏省高校自然科学基因项目(18KJA230002);淮安市精准农业技术工程实验室(HAP201703);江苏省“333工程”高层次人才培养项目(2016);江苏省高校优秀中青年教师和校长赴境外研修项目(2016)。

摘　　要：为研究LY294002和AG-490对mTOR和JAK-STAT信号通路的抑制作用,本试验以泌乳期Wistar大鼠为试验动物,对新生仔鼠的平均日增重、泌乳大鼠平均产奶量、大鼠乳腺上皮细胞形态学变化、血浆和乳腺组织中激素和蛋白基因的表达等指标进行测定。结果表明:在正常生理水平条件下,外源激素和信号通路抑制剂均抑制了乳酪蛋白的合成,这给泌乳蛋白合成的外源调控提供了重要的实践依据。In order to study the effect of inhibitors LY294002 and AG-490 on mTOR and JAK-STAT signal pathway,the healthy lactation Wistar rats were choosed to study,and then,the newborn rats average daily gain,the mother rats average milk production,the rats mammary gland morphology changes,the hormone level in blood plasma and breast tissue,the protein genes expression were determined.The results showed that the exogenous hormones and signaling pathway inhibitors inhibited the synthesis of casein under rats normal physiological conditions,which provided an important practical basis for the use of exogenous regulation of lactation protein synthesis.

关 键 词：激素 抑制剂 乳腺组织形态 乳蛋白 

分 类 号：S811[农业科学—畜牧学]