基于同步移相显微干涉的血红细胞形貌测量方法  

Profile measurement of red blood cells based on simultaneous phase-shifting microscopic interference

作　　者：徐君宜 袁群[1] 高志山[1] 徐尧 XU Junyi;YUAN Qun;GAO Zhishan;XU Yao(School of Electronic and Optical Engineering,Nanjing University of Science and Technology,Nanjing 210094,China;Jiangsu Shuguang Photoelectric Co.,Ltd.,Yangzhou 225109,China)

机构地区：[1]南京理工大学电子工程与光电技术学院,江苏南京210094 [2]江苏曙光光电有限公司,江苏扬州225109

出　　处：《应用光学》2020年第5期1020-1025,共6页Journal of Applied Optics

基　　金：国家自然科学基金(U1931120);中央高校基本科研业务专项(30917011107,30918014115-004,30919011277)。

摘　　要：血红细胞的形貌特征是医学领域对多种疾病进行预防和诊断的一项重要指标,提出一种同步移相显微干涉法实现对血红细胞形貌的动态测量。搭建了透射式显微干涉成像系统,测量了100μm内径模拟微血管内、名义直径为7μm^8μm、高度最大值为2μm的新西兰兔血红细胞,针对血红细胞所处的微血管环境提出了基于微血管相位相减的血红细胞形貌提取方法和成像放大率校正方法,实验得到模拟微血管内的血红细胞平均直径7.757μm和平均最高高度2.022μm,验证了本方法具有在体定量测量血红细胞形貌的潜力。The profile of red blood cells is an important index for the prevention and diagnosis of various diseases in the medical field.A simultaneous phase-shifting microscopic interference method was proposed to realize the dynamic measurement of the profile of red blood cells.A transmission-type microscopic interference imaging system was set up to measure the red blood cells of New Zealand rabbit with a diameter of 7μm^8μm,and a maximum height of 2μm in a simulated microvessel with an inner diameter of 100μm.Aiming at the microvesse environment in which the red blood cells were located,a method of extracting the profile of red blood cells based on the phase subtraction of microvessels and a correction method of imaging magnification were proposed.The average diameter and maximum height of the red blood cells in the simulated microvessels are obtained by experiments,which are 7.757μm and 2.022μm,respectively.The potential of this method in vivo measurement of RBC profile has been verified.

关 键 词：形貌测量 同步移相 血红细胞形貌 显微干涉 

分 类 号：TN202[电子电信—物理电子学] TN209