牛磺酸诱导人结直肠癌细胞增殖、凋亡的作用机制分析  

Action mechanism of taurine on inducing proliferation and apoptosis of human colorectal cancer cells

作　　者：张月凡[1] 杨桂彬[2] 张天文 ZHANG Yue-fan;YANG Gui-bin;ZHANG Tian-wen(Aerospace Center Hospital,Hematology,100049;Aerospace Center Hospital,Gastroenterology,100049)

机构地区：[1]航天中心医院血液科,100049 [2]航天中心医院消化科,100049

出　　处：《现代消化及介入诊疗》2020年第8期1051-1054,共4页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：河北省医药卫生科技计划(H2018652);河北省沧州市科学技术研究与发展指导计划(172302047)。

摘　　要：目的分析牛磺酸诱导人结直肠癌细胞增殖、凋亡的作用机制。方法在体外用不同浓度(100、200、400 m M)的牛磺酸处理人结肠癌Caco-2细胞,以空白组(0 m M牛磺酸)为对照组,MTT法检测牛磺酸对Caco-2细胞增殖活性的影响,Hoechest 33258染色法检测牛磺酸对Caco-2细胞凋亡的影响,Western blot检测牛磺酸对Caco-2细胞Mst1、JNK蛋白表达及JNK磷酸化的影响。结果与空白组相比,不同浓度牛磺酸处理后Caco-2细胞增殖抑制率显著升高,且牛磺酸浓度越高、处理时间越长,对Caco-2细胞增殖活性的抑制作用越明显,差异有统计学意义(P<0.05)。与空白组相比,不同浓度牛磺酸处理后Caco-2细胞凋亡率显著升高,Caco-2细胞中Bcl-2/Bax比率凋亡率显著降低,均呈浓度依赖,差异有统计学意义(P<0.05)。经不同浓度牛磺酸处理后Caco-2细胞中,Mst1、p-JNK蛋白表达显著升高,差异有统计学意义(P<0.05)。结论牛磺酸可能通过Mst1-JNK信号通路抑制人结直肠癌细胞增殖,促进凋亡。Objective To analyze the action mechanism of taurine on inducing proliferation and apoptosis of human colorectal cancer cells.Methods Human colon cancer Caco-2 cells were treated with taurine of different concentrations(100,200,400 m M)in vitro.The blank group(0 m M)was set up as the control.The effects of taurine on proliferation activity of Caco-2 cells were detected by MTT assay.Hoechest 33258 staining was applied to detect effects of taurine on apoptosis of Caco-2 cells.Western blot was applied to detect effects of taurine on expression of Mst1 and JNK proteins,and JNK phosphorylation in Caco-2 cells.Results Compared with blank group,after different-concentration taurine treatment,proliferation inhibition rate of Caco-2 cells was significantly increased.The higher the taurine concentration and the longer the treatment time,the more significant the inhibition effects on proliferation activity of Caco-2 cells(P<0.05).Compared with blank group,after different-concentration taurine treatment,apoptosis rate of Caco-2 cells was significantly increased,Bcl-2/Bax and apoptosis rate in Caco-2 cells were significantly decreased,showing concentrationdependence(P<0.05).After different-concentration taurine treatment,expression of Mst1 and p-JNK proteins in Caco-2 cells was significantly increased(P<0.05).Conclusion Taurine may inhibit proliferation of human colorectal cancer cells and promote their apoptosis by Mst1-JNK signaling pathway.

关 键 词：结直肠癌 牛磺酸 细胞增殖 细胞凋亡 

分 类 号：R735.35[医药卫生—肿瘤] R574[医药卫生—临床医学]