Regular moderate aerobic exercise improves high-fat diet-induced nonalcoholic fatty liver disease via monoacylglycerol O-acyltransferase 1 pathway suppression  

作　　者：Kyung-Wan Baek Jeong-An Gim Jung-Jun Park 

机构地区：[1]Division of Sport Science,Pusan National University,Busan 46241,Korea [2]Department of Biological Sciences,College of Natural Sciences,Pusan National University,Busan 46241,Korea

出　　处：《Journal of Sport and Health Science》2020年第5期472-478,共7页运动与健康科学（英文）

摘　　要：Purpose:Monoacylglycerol O-acyltransferase 1(MGAT1)is reported to play a key role in the development of diet-induced nonalcoholic fatty liver disease(NAFLD).Thus,this study investigated the effect of exercise on suppression of the MGAT1 pathway in NAFLD tissue of high-fat diet(HFD)-induced obese rats.Methods:Male Sprague-Dawley rats were fed an HFD containing 45%fat for 6 weeks.Upon confirmation that NAFLD had been induced in the obese animals,they were divided into HFD-fed groups provided with exercise(HFD+EXE)or without exercise(HFD)and a group given dietary adjustment(DA)only,for a further 6 weeks of intervention treatment.The 6-week regular moderate aerobic exercise consisted of an accommodation phase with increasing exercise.Lipid accumulation in the liver tissue was determined by Oil Red O staining.The MGAT1 and liver lipogenic gene mRNA levels were measured by qPCR,and their protein levels by western blot assay.Results:Oil Red O staining showed that NAFLD was successfully induced by HFD-fed.The gene expression of MGAT1 was significantly lower in HFD+EXE than HFD.However,there was no significant difference between HFD+EXE and DA.The protein expression of MGAT1 was significantly lower in HFD+EXE than both HFD and DA.Messenger RNA and protein expression of other lipogenic genes were not different among groups.These data indicate that exercise suppresses MGAT1 pathway regardless of HFD feeding;in part,this effect could be greater than DA.Conclusion:Our data suggest that exercise can improve NAFLD,which is probably due to suppression of MGAT1 pathway.

关 键 词：EXERCISE High-fat diet Monoacylglycerol O-acyltransferase 1 Nonalcoholic fatty liver disease OBESITY 

分 类 号：G804.2[文化科学—运动人体科学]