纳滤法制备高纯度雪莲果低聚果糖及其结构表征  

Preparation of High Purity Yacon Fructo-oligosaccharides by Nanofiltration and Its Characterization

作　　者：曲晓萌 杨革[2] 张泽浩 张兰[4] QU Xiaomeng;YANG Ge;ZHANG Zehao;ZHANG Lan(Shandong Province Jining Health School,Jining 272000,China;College of Life Science,Qufu Normal University,Qufu 273165,China;Shandong First Medical University,Tai’an 271000,China;Mudanjiang Medical University,Mudanjiang 157011,China)

机构地区：[1]山东省济宁卫生学校,山东济宁272000 [2]曲阜师范大学生命科学学院,山东曲阜273165 [3]山东第一医科大学,山东泰安271000 [4]牡丹江医学院,黑龙江牡丹江157011

出　　处：《生物质化学工程》2020年第5期37-44,共8页Biomass Chemical Engineering

基　　金：贵州省科技攻关项目(黔科合S字[2007]1017)。

摘　　要：采用纳滤分离技术以雪莲果为原料纯化低聚果糖,通过Box-Behnken中心组合设计(CCD)及响应面分析(RSM)建立预测低聚果糖纯度的二次多项数学模型,优化分离纯化工艺。结果表明:操作压力0.15 MPa,循环流量5.3 mL/min,pH值2.7时,纯化倍数为5,实际低聚果糖纯度为95.1%,达到P级产品标准。通过应用HPLC-MS、IR、1H NMR及13C NMR等对雪莲果低聚果糖组分的纯度、组成、结构进行表征,结果表明:分离纯化得到的雪莲果低聚果糖由蔗果三糖、蔗果四糖和蔗果五糖组成,纯度为95.1%,平均包含5个果糖单元,主要是由β-呋喃构型的果糖组成。The yacon fructo-oligosaccharides(FOS)was separated and purified by the nanometer membrane from yacon.A quadratic mathematical model for predioting the concentration of oligosaccharides was developed to optimize the purification process by Box-Behnken central composite design(CCD)and response surface methodology(RSM).Consequently,the optimized processing parameters were determined as operating pressure 0.15 MPa,circulation flow rate 5.3 mL/min,pH value 2.7,purification fold 5,the purity of the obtained FOS was 95.1%,which could reach P grade standard.HPLC-MS,IR,1H NMR and 13C NMR were used to characterize the purity,composition and structure of FOS.The results showed that the purified FOS was composed of kestose,nystose and bestonentaose.The purity was 95.1%and it contained on average of 5 fructose units.The yacon FOS was mainly composed ofβ-furan-fructose tested by IR.

关 键 词：雪莲果 低聚果糖 纳滤 分离纯化 结构表征 

分 类 号：TQ35[化学工程]