不同血清型腺相关病毒载体转染小鼠视网膜后的表达效率  

Expression pattern of different serotypes of adeno-associated viral vectors in mouse retina

作　　者：胡双 杨丽萍[1] HU Shuang;YANG Li-ping(Department of Ophthalmology, Peking University Third Hospital, Beijing 100191, China)

机构地区：[1]北京大学第三医院眼科,北京100191

出　　处：《北京大学学报（医学版）》2020年第5期845-850,共6页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(81470666,81770966)。

摘　　要：目的:研究不同血清型腺相关病毒(adeno-associated virus,AAV)载体介导的外源基因在视网膜中的表达效率,同时比较AAV载体和两种眼科常用启动子组合后转染小鼠视网膜的表达效率高低,为视网膜色素变性基因治疗选择合适的AAV载体与启动子提供依据。方法:AAV病毒根据衣壳蛋白不同可分为不同血清型,本课题选取视网膜疾病基因治疗中常用的AAV2/2、AAV2/5、AAV2/8和AAV2/9四种血清型AAV载体,并以绿色荧光蛋白(green fluorescent protein,GFP)作为报告基因,用GFP的表达强度判断AAV载体介导的外源基因在视网膜中的表达效率。AAV载体纯化后滴度为1.00×1013 mg/L,注射1μL至C57BL/6J小鼠视网膜下腔,于2周取眼球做成冰冻切片,在共聚焦显微镜下观察GFP在小鼠视网膜各层的表达情况。选取在感光细胞内特异性表达最强的AAV2/8于第4周取眼球冰冻切片继续观察是否能持续稳定表达。随后选取眼科基因治疗最常用的广谱启动子CMV和由CMV增强子与鸡β-肌动蛋白启动子组成的CAG启动子,并构建AAV2/8-GFP-CMV和AAV2/8-GFP-CAG两种不同启动子的病毒载体注射至视网膜下腔,于2周取眼球做成冰冻切片,在共聚焦显微镜下观察不同启动子的AAV2/8在小鼠视网膜各层的表达情况。结果:注射AAV-GFP后未见典型的术后细菌感染及明显免疫反应。AAV2/2、AAV2/5、AAV2/8和AAV2/9四种血清型AAV载体视网膜下腔注射2周后,AAV2/8和AAV2/9在小鼠视网膜的GFP绿色荧光明显,说明这两种AAV载体转染小鼠视网膜后的表达效率高,而在这两种血清型中,AAV2/8的GFP绿色荧光主要集中在感光细胞内,AAV2/9在视网膜全层均有表达,说明AAV2/8对视网膜感光细胞特异性更强。对AAV2/8的进一步实验表明在视网膜下腔注射4周后小鼠视网膜的GFP绿色荧光明显,说明AAV2/8载体介导的外源基因能在体内稳定表达。使用CMV启动子时GFP在感光细胞与视网膜色素上皮细�Objective:To investigate the expression efficiency of exogenous gene mediated by different serotypes of adeno-associated virus(AAV)vectors in retina,and to compare the expression efficiency of AAV vector and two kinds of promoters commonly used in ophthalmology after transfection into mouse retina,so as to provide the basis for selecting appropriate AAV vector and promoter for gene therapy of retinitis pigmentosa.Methods:AAV2/2,AAV2/5,AAV2/8 and AAV2/9 were prepared.The C57BL/6J mice were injected subretinally with 1μL purified AAV vectors(1.00×1013 mg/L).Then the mice were killed 2 or 4 weeks after treatment,and the eyes were enucleated for frozen section.The expression of green fluorescent protein(GFP)was observed under the confocal microscope.Two kinds of promoters,CMV and CAG,were selectd,and the expression of AAV2/8-GFP-CMV and AAV2/8-GFP-CAG was observed under confocal microscope.Results:No bacterial infection or immune response were seen in the injected mice.2 weeks after injection,the GFP green fluorescence of AAV2/8 and AAV2/9 in the mouse retina was obvious,which indicated that the GFP green fluorescence of AAV2/8 and AAV2/9 was high after transfection into the mouse retina.In these two serotypes,GFP green fluorescence of AAV2/8 was mainly concentrated in photoreceptor cells while AAV2/8 was expressed in the whole retina,indicating that AAV2/8 was more specific to photoreceptors.Further experiments on AAV2/8 showed that the GFP green fluorescence of the mouse retina was obvious 4 weeks after injection,indicating that the exogenous gene mediated by AAV2/8 could be stably expressed in vivo.For CMV and CAG promoters,CMV promoter was expressed stronger in retinal pigment epithelium(RPE)cells,while CAG promoter was stronger in photorecepters.In photorecepters,CAG promoter was expressed almost the same as CMV promoter,while CMV promoter was stronger in RPE cells.Conclusion:AAV vectors could express transgene robustly in retinal cells;Among several AAV serotypes,AAV2/2 and AAV2/5 showed weaker GFP fluorescen

关 键 词：腺相关病毒 视网膜色素变性 基因治疗 

分 类 号：R774.1[医药卫生—眼科]