2种食用菌NADPH氧化酶A蛋白结构分析  

Analysis of the NADPH Oxidase A(NoxA)Structure Characteristics of Two Edible Fungi

作　　者：李银凤[1] 刘晓柱[1] LI Yin-feng;LIU Xiao-zhu(Guizhou Institute of Technology,Guiyang 550000,China)

机构地区：[1]贵州理工学院,贵州贵阳550003

出　　处：《中国食用菌》2020年第8期22-28,共7页Edible Fungi of China

基　　金：2020年贵州省科技计划项目(黔科合基础[2020]1Y057)。

摘　　要：NADPH氧化酶A(NADPH oxidase A,Nox A)为NADPH氧化酶亚家族一员,可催化产生ROS。通过检索Gen Bank中Nox A序列,利用生物信息学方法分析了2种食用菌Nox A蛋白结构特征。结果表明,在Gen Bank中仅搜索到平菇Nox A(Po Nox A)与双色蜡蘑Nox A(Lb Nox A)序列。进一步序列比对发现,2种Nox A序列同源性达80%,均包含2个FAD结合模体、4个NADPH结合模体以及4个亚铁红素结合位点(heme binding)中的保守组氨酸残基;蛋白结构分析表明,2种Nox A一级结构、二级结构及三级结构较为相似,但在结构数量上也存在一定差异;2种Nox A均属于跨膜蛋白,但Po Nox A蛋白N端存在5个跨膜结构,Lb Nox A蛋白N端存在6个跨膜结构,中间区域存在1个跨膜结构;2种Nox A蛋白质中均存在磷酸化位点修饰,具有潜在的磷酸激酶特性。但2种Nox A蛋白质中丝氨酸、苏氨酸及酪氨酸修饰位点个数和存在位置不同;进化分析表明,2种Nox A基因亲缘关系最近,位于进化树的同一分枝。因此推测2种食用菌Nox A氧化酶功能较一致,但也存在差异性。NADPH oxidase A,one member of the NADPH oxidase subfamily,catalyzes the production of ROS.The Nox A sequences from GenBank were searched.The Nox A structure characteristics differences between two edible fungi species were analyzed using bioinformatics methods.Searching results indicated that only Po Nox A and LbNoxA were retrieved from GenBank database.Sequence analysis demonstrated that Po Nox A shared 80%homologies with LbNoxA.There were two FAD binding motifs,four NADPH binding motifs and four conserved histidine residues involved in heme binding were found in these two Nox A.Information analysis showed that the first,secondary and tertiary structures of Nox A were similar,but some differences in the structure numbers still existed between Po Nox A and LbNoxA.In addition,Po Nox A and LbNoxA were transmembrane proteins,and five transmembrane domains were found in the N-terminal of Po Nox A.However,six transmembrane domains and one transmembrane domain were discovered in the N-terminal and the middle of LbNoxA,respectively.Furthermore,phosphorylation sites were also detected in these two Nox A,and had potential phosphokinase properties.But the number and location of serine,threonine and tyrosine modification sites in Po Nox A and LbNoxA were different.Evolutionary analysis showed that Po Nox A and LbNox A shared closely genetic relationship,and on the same branch of evolutionary tree.Therefore,it is speculated that the functions of Nox A of these two edible fungi were relatively consistent,but differences also existed.

关 键 词：食用菌 NADPH氧化酶A 蛋白结构 

分 类 号：S646.9[农业科学—蔬菜学]