罗非鱼鱼皮胶原蛋白酶法提取工艺及特性研究  

Study on Extraction Process and Characteristics of Collagen from Tilapia Skin by Enzymatic Method

作　　者：王晴 钱玉梅[1] 李红侠[1] 陈红玲[1] WANG Qing;QIAN Yumei;LI Hongxia;CHEN Hongling(Suzhou University,Suzhou 234000,Anhui;Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food,Suzhou 234000,Anhui)

机构地区：[1]宿州学院,安徽宿州234000 [2]宿州市天然产物与功能性食品工程技术研究中心,安徽宿州234000

出　　处：《攀枝花学院学报》2020年第5期6-10,共5页Journal of Panzhihua University

基　　金：宿州学院科研平台开放课题项目(2017ykf05、2019ykf28);安徽省高校自然科学研究重点项目(KJ2018A0443、KJ2017A441);宿州学院学术技术带头人项目(2016XJXS06);宿州学院宿州市农业科学院实践教育基地(szxy2018xxhz02);宿州学院自然科学重点项目(2017yzd11);安徽省自然科学基金项目(1908085MC100);宿州学院教授博士科研启动基金项目(2016jb02)。

摘　　要：采用单因素和正交试验优化了胃蛋白酶法提取罗非鱼鱼皮的酶解条件,并探究其溶解性能及保水性能。结果表明:酶解时间15 h、酶解温度50℃、料液比1:60、pH为2时,胶原蛋白提取率最高,为90.3%,该法提取的胶原蛋白溶解性能及保水性能较好,且在酸性条件下溶解性能更好,可为胶原蛋白的进一步开发及应用提供理论依据。This paper adopts single factor and orthogonal test to optimize enzymatic hydrolysis conditions of tilapia skin and investigates its solubility as well as its water retention.The results showed that when the time of enzymatic hydrolysis was 15 h,the enzymatic hydrolysis temperature was 50℃,the solid-liquid ratio was 1:60,and the pH was 2,the extraction rate of collagen was the highest at 90.3%.The collagen extracted by this method exhibited better solubility and water retention,and better solubility under acidic conditions,which could provide theoretical basis for the further development and application of collagen.

关 键 词：胶原蛋白 胃蛋白酶 鱼皮 理化特性 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]