壳寡糖对体外诱导的非酒精性脂肪肝细胞模型的降脂机理研究  

Study on Lipid-Lowering Mechanism of Chitooligosaccharides on Non-Alcoholic Fatty Liver Cell Model

作　　者：沈欣 赵梦瑶 邱勇隽[1] 夏泉鸣[1] 赵黎明[1] SHEN Xin;ZHAO Mengyao;QIU Yongjun;XIA Quanming;ZHAO Liming(School of Bioengineering, East China University of Science and Technology, Shanghai 200237, China)

机构地区：[1]华东理工大学生物工程学院,上海200237

出　　处：《食品科学技术学报》2020年第5期41-47,77,共8页Journal of Food Science and Technology

基　　金：国家重点研发计划课题(2017YFB0309302,2019YFD0901805)。

摘　　要：为探讨壳寡糖在体外对肝脂质积累的抑制效果及潜在作用机制,选用油酸钠诱导HepG2细胞建立非酒精性脂肪肝细胞模型,通过油红O染色及各试剂盒检测胞内总脂质积累、甘油三酯、总胆固醇、高低密度脂蛋白胆固醇及游离脂肪酸的含量,实时定量PCR及western blot检测细胞内脂代谢相关基因和蛋白的表达。结果发现,4.0 mg/mL的壳寡糖对肝脂质蓄积具有明显的抑制作用,并降低了二酰基甘油酰基转移酶2(DGAT2)、肝X受体α(LXRα)、过氧化物酶体增殖物激活受体-γ(PPARγ)、孕烯醇酮X受体(PXR)和分化簇36(CD36)的mRNA水平和蛋白水平的表达。研究结果表明,壳寡糖可以通过调节脂肪酸吸收和甘油三酯合成通路从而降低非酒精性脂肪肝细胞模型中的脂质积累。希望本研究能够为利用壳寡糖开发具有降脂护肝功能的膳食补充剂提供理论参考。To investigate the effect and potential mechanism of chitooligosaccharides on reducing liver lipid accumulation in vitro,sodium oleate was used to induce HepG2 cells to establish non-alcoholic fatty liver cell model,the lipid profiles including total lipid accumulation,total triglyceride,total cholesterol,high/low density lipoprotein cholesterol and free fatty acids contents were analyzed by oil red O staining and corresponding commercial kits,respectively.The expression of genes and proteins related to intracellular lipid metabolism were detected by real-time PCR and western blot.The results indicated that 4.0 mg/mL chitooligosaccharides had a significant inhibitory effect on liver lipid accumulation and reduced the mRNA and protein expression levels of diacylglycerol acyltransferase 2(DGAT2),liver X receptor alpha(LXRα),peroxisomes proliferator-activated receptor-γ(PPARγ),pregnenolone X receptor(PXR)and differentiation cluster 36(CD36).These results suggested that chitooligosaccharides could reduce the accumulation of lipids by regulating fatty acid absorption and triglyceride synthesis pathways.This study aims to provid theoretical support for using chitooligosaccharides as dietary supplements in lipid-lowering and liver protection.

关 键 词：壳寡糖 非酒精性脂肪肝 细胞模型 降脂机理 CD36 DGAT2 

分 类 号：TS201.4[轻工技术与工程—食品科学] Q539[轻工技术与工程—食品科学与工程]