A 3D biophysical model for cancer spheroid cell-enhanced invasion in collagen-oriented fiber microenvironment  

作　　者：Miaomiao Hai Yanping Liu Ling Xiong Guoqiang Li Gao Wang Hongfei Zhang Jianwei Shuai Guo Chen Liyu Liu 海苗苗;刘艳平;熊玲;李国强;王高;张鸿飞;帅建伟;陈果;刘雳宇(Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials,College of Physics,Chongqing University,Chongqing 401331,China;Hygeia International Cancer Hospital,Chongqing 401331,China;Department of Physics,Xiamen University,Xiamen 361005,China)

机构地区：[1]Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials,College of Physics,Chongqing University,Chongqing 401331,China [2]Hygeia International Cancer Hospital,Chongqing 401331,China [3]Department of Physics,Xiamen University,Xiamen 361005,China

出　　处：《Chinese Physics B》2020年第9期581-588,共8页中国物理B（英文版）

基　　金：Project supported by the National Natural Science Foundation of China(Grant Nos.11974066 and 11674043);the Fundamental Research Funds for the Central Universities,China(Grant No.2019CDYGYB007);the Natural Science Foundation of Chongqing,China(Grant No.cstc2019jcyj-msxmX0477).

摘　　要：The process of in situ tumors developing into malignant tumors and exhibiting invasive behavior is extremely complicated.From a biophysical point of view,it is a phase change process affected by many factors,including cell-to-cell,cell-to-chemical material,cell-to-environment interaction,etc.In this study,we constructed spheroids based on green fluorescence metastatic breast cancer cells MDA-MB-231 to simulate malignant tumors in vitro,while constructed a three-dimensional(3D)biochip to simulate a micro-environment for the growth and invasion of spheroids.In the experiment,the 3D spheroid was implanted into the chip,and the oriented collagen fibers controlled by collagen concentration and injection rate could guide the MDA-MB-231 cells in the spheroid to undergo directional invasion.The experiment showed that the oriented fibers greatly accelerated the invasion speed of MDA-MB-231 cells compared with the traditional uniform tumor micro-environment,namely obvious invasive branches appeared on the spheroids within 24 hours.In order to analyze this interesting phenomenon,we have developed a quantitative analyzing approach to explore strong angle correlation between the orientation of collagen fibers and invasive direction of cancer cell.The results showed that the oriented collagen fibers produced by the chip can greatly stimulate the invasion potential of cancer cells.This biochip is not only conducive to modeling cancer cell metastasis and studying cell invasion mechanisms,but also has the potential to build a quantitative evaluation platform that can be used in future chemical drug treatments.

关 键 词：3D biochip SPHEROIDS MDA-MB-231 cells oriented collagen fibers cancer cell invasion 

分 类 号：TN492[电子电信—微电子学与固体电子学] R737.9[医药卫生—肿瘤]