高脂饮食对大鼠主动脉内皮细胞形态及刚度的影响  

Effects of High-Fat Diet on Rat Aortic Endothelial Cell Morphology and Stiffness

作　　者：伍诚麟 郭嘉馨 杨佳丽 张伟宸 邓小燕[1,2] 康红艳[1,2] WU Chenglin;Guo Jiaxin;YANG Jiali;ZHANG Weichen;DENG Xiaoyan;KANG Hongyan(Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education,School of Biological Scienceand Medical Engineering,Beihang University,Beijing 100083,China;Advanced Innovation Center for Biomedical Engineering,Beihang University,Beijing 100083,China)

机构地区：[1]北京航空航天大学生物与医学工程学院生物力学与力生物学教育部重点实验室,北京100083 [2]北京航空航天大学生物医学工程高精尖创新中心,北京100083

出　　处：《医用生物力学》2020年第4期317-322,共6页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(31870940,11772036,11572028)。

摘　　要：目的研究高脂饮食对大鼠主动脉内皮细胞形态与刚度的影响。方法将SD大鼠随机分为高脂饲养组(AS组,n=3)与对照组(CON组,n=3),利用植块法获得大鼠胸主动脉内皮细胞。在倒置显微镜下观察细胞形态,通过荧光染色比较两组细胞F-actin平均荧光强度,利用原子力显微镜(atomic force microscopy,AFM)检测细胞刚度。结果细胞在植块后第7 d有少量细胞爬出,第14 d细胞融合成单层,利用Ⅷ因子免疫荧光染色法证实为内皮细胞。高脂饮食引起细胞周长(P<0.01)、长轴/短轴比率(P<0.01)、面积增大(P>0.05);细胞圆度减小(P<0.01)。高脂组F-actin平均荧光强度明显高于对照组(P<0.01)。AFM显示高脂饲养引发内皮细胞刚度显著增大(P<0.01)。结论高脂饮食将引发内皮细胞形态与刚度发生改变,进而影响血管正常功能,使其成为动脉粥样硬化的重要诱因。Objective To investigate the high-fat diet effect on morphology and stiffness of endothelial cells.Methods SD rats were randomly divided into high-fat diet group (AS group,n = 3) and control group (CON group,n = 3). Rat aortic endothelial cells were obtained from rat thoracic aorta by explant method. Cell morphology was observed under inverted microscopy. The mean fluorescent intensity of F-actin in two groups was calculated by immunofluorescence staining. Cell stiffness was measured by atomic force microscopy (AFM).Results The endothelial cells migrated from tissue plant on the 7 thday and formed confluence after cultivation for 14 days. Endothelial cells were identified by factor Ⅷ immunofluorescence staining. Cells in AS group showed enhanced perimeter (P< 0. 01),aspect ratio (P< 0. 01),and area (P> 0. 05),while less circularity (P < 0. 01)compared with the cells in control group. The mean fluorescence intensity of F-actin in AS group was significantly higher than that in control group (P<0. 01). AFM showed that the cell stiffness of AS group was significantly higher than that of control group (P < 0. 01). Conclusions High-fat diet would change the morphology and stiffness of endothelial cells,which might subsequently affect their normal function and become an important incentive to AS.

关 键 词：高脂饮食 血管内皮细胞 刚度 原子力显微镜 

分 类 号：R318.01[医药卫生—生物医学工程]