蛋白质组学在生鲜肉肉色变化机制研究中的应用  

Application of proteomics in mechanism research of fresh meat color changes

作　　者：梁荣蓉[1] 许宝琛 张一敏[1] 杨啸吟[1] 朱立贤[1] 毛衍伟[1] 张文华 罗欣[1] Liang Rongrong;Xu Baochen;Zhang Yimin;Yang Xiaoyin;Zhu Lixian;Mao Yanwei;Zhang Wenhua;Luo Xin(College of Food Science and Engineering,Shandong Agricultural University,Taian 271018,China;National Beef Cattle Industrial Technology System,Zhongwei Station,Zhongwei 755000)

机构地区：[1]山东农业大学食品科学与工程学院,泰安271018 [2]国家肉牛牦牛产业技术体系中卫试验站,中卫755000

出　　处：《农业工程学报》2020年第16期283-292,共10页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金面上项目(31871731);山东省重点研发计划项目(2019GNC106050);国家肉牛牦牛产业技术体系建设专项项目(CRAS-37);山东省现代农业产业技术体系创新团队建设专项(SDAIT-09-09);国家自然科学基金青年项目(31901757)。

摘　　要：肉色是影响消费者购买欲望的最直观因素。宰后生鲜肉肉色的变化机制和肉色改善一直是肉类科学领域研究的热点。肉色体系复杂,其稳定性受动物宰前因素、从肌肉向可食用肉转化过程和宰后商业流通链条件等因素的影响。目前,仍有许多影响途径和作用机制不够清楚。研究者已采用蛋白质组学技术发现了许多与肉色相关的蛋白标志物和可能影响途径。该文基于近年的研究,综述了不同处理条件下与肉色相关的差异蛋白,主要包括结构性蛋白中的肌动蛋白、肌球蛋白和肌联蛋白,以及肌浆蛋白中的伴侣蛋白、热休克蛋白、代谢酶类和氧化还原酶类等,分析了差异蛋白表达对肉色的影响及其表达调控肉色体系的可能机制,为今后肉色变化机制的研究和肉色稳定性的调控提供了思路。Meat color is a major quality attribute that influences the purchasing decisions of consumers at the point of sale.The stability of meat color gradually decreases after slaughter which then eventually leads to the discoloration.The discoloration will result in lost value during retail display.So,the mechanism behind slaughtering meat color variation is always a hot topic in the field of meat science which has been widely studied in order to improve meat color stability.Many factors from both preharvest and postharvest can influence the color stability,including pH value,temperature,different muscle,package,lipid oxidation,processing and so on.However,in this complex system,the inherent factors that determine meat color include the microstructure changes and biochemical functions.The structural protein determines the micro spatial structure of muscle,which affects the meat color from the aspects of water holding capacity,light scattering,and projection,etc.Enzymes and chaperones in sarcoplasmic protein mainly represent meat color by maintaining intracellular homeostasis,and regulate metabolism and myoglobin status.So far,the effects of many factors on meat color have been described in detail,and the role of myoglobin in meat color has been much clear.However,the appearance of meat color is a complex biochemical system,and there are still many unknowns to be explored.What happens to myoglobin and other functional proteins in this complex system remains unknown.Recently,many protein markers have been found in the application of proteomics in meat color research,and the pathways related to these biomarkers have been highlighted.However,that is still far from enough to clearly understand the variable color changes.The complex interaction between myoglobin and other functional proteins or enzymes are not fully understood.Therefore,the present article reviews the major achievements of recent proteomic researches on meat color of the biological functions and further summarized the potential mechanism of fresh meat discol

关 键 词：肉 颜色 肌红蛋白 蛋白组学 差异蛋白 伴侣蛋白 代谢酶类 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]