利玛原甲藻中大田软海绵酸和鳍藻毒素-1提取与纯化  

Extraction and purification of okadaic acid(OA) and dinophysis toxin-1(DTX1) in the dinoflagellate Protocentrum Lima

作　　者：金薇 姚敬元 陈文博 宫长宝 贾宝林 隋世峰 冯少姝 韩蕾 梁玉波[2,3] 吕颂辉 JIN Wei;YAO Jing-yuan;CHEN Wen-bo;GONG Chang-bao;JIA Bao-lin;SUI Shi-feng;FENG Shao-shu;HAN Lei;LIANG Yu-bo;LV Song-hui(Jinan University,Guangzhou 510632,China;National Marine Environmental Monitoring Center,Dalian 116023,China;Dalian key laboratory of algal toxin.Dalian 116023,China;Dalian modem agricultural production development service center,Dalian 116023,China;Dalian University of Technology,Dalian 116024,China)

机构地区：[1]暨南大学,广东广州510632 [2]国家海洋环境监测中心,辽宁大连116023 [3]大连市藻毒素重点实验室,辽宁大连116023 [4]大连市现代农业生产发展服务中心,辽宁大连116023 [5]大连理工大学,辽宁大连116024

出　　处：《海洋环境科学》2020年第5期791-797,共7页Marine Environmental Science

基　　金：国家重点研发计划(2016YFF0201104,2017FYC1404303);国家自然科学基金(41576120,41276099);国家海洋局海洋赤潮灾害立体监测技术与应用重点实验室开放基金(MAYTHAB201605);国家海洋局近岸海域生态环境重点实验室开发基金(201613)。

摘　　要：批量培养产生腹泻性贝类毒素的利玛原甲藻,甲醇提取超声破碎后的藻细胞;用大孔吸附树脂HP20富集其胞外毒素并进行毒素的初步纯化。经制备型高效液相色谱法分离,分别得到毫克级的大田软海绵酸和鳍藻毒素-1固体。经核磁共振氢谱和高分辨质谱确认结构,定量核磁确定纯度,得到的大田软海绵酸和鳍藻毒素-1纯度分别为99.39%和99.26%,满足标准物质制备要求。Marine dinoflagellate Prorocentrum lima producing diarrhetic shellfish poison were mass cultured and crushed by ultrasonic, followed by extracting with methanol for okadaic acid(OA) and dinophysis toxin-1(DTX1). OA and DTX1 with high purity were obtained by a preparative high performance liquid chromatographic(HPLC) method after enrichment and purification with macroporous adsorption resin HP20,which was used for the enrichment of extracellular toxin secreted in the sea water. The two compounds were identified by nuclear magnetic resonance hydrogen spectrum(1 H-NMR) and high-resolution mass spectrometry(HRMS). The purity of OA and DTX1 was identified by quantitative nuclear magnetic to be 99.39% and99.26% respectively, meeting the requirements for preparation of standard material.

关 键 词：利玛原甲藻 大田软海绵酸 鳍藻毒素-1 提取 纯化 

分 类 号：Q-331[生物学] X171.5[环境科学与工程—环境科学]