Transformable peptide nanoparticles inhibit the migration of N-cadherin overexpressed cancer cells  

作　　者：Jiaqi Fan Yu Fan Zijin Wei Yijing Li Xiangdan Li Lei Wang Hao Wang 

机构地区：[1]Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education&Hubei Key Laboratory of Catalysis and Materials Science,South-Central University for Nationalities,Wuhan 430074,China [2]GAS Center for Excellence in Nanoscience,CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,National Center for Nanoscience and Technology(NCNST),Beijing 100190,China

出　　处：《Chinese Chemical Letters》2020年第7期1787-1791,共5页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(Nos.51890891,51725302,21807020,51573031 and 51573032);National Key R&D Program of China(No.2018YFE0205400);Science Fund for Creative Research Groups of the National Natural Science Foundation of China(No.11621505);CAS Interdisciplinary Innovation Team。

摘　　要：About 90%cancer-related mortality results from the cancer metastasis,which generally undergoes after epithelial-mesenchymal transition(EMT)process.N-Cadherin,overexpressed on cancer cell surface during EMT,can enhance the migration of cancer cells.He rein,we design and synthesize a transformable peptide BP-KLVFF-SWTLYTPSGQSK(BFS)that can block N-cadherin for inhibiting cancer migration and metastasis.The peptide BFS consists of three modules including(1)the hydrophobic bis-pyrene(BP)unit for forming and locating nanoparticles,(2)the KLVFF peptide sequence for forming and stabilizing fibrous structures and(3)the targeting peptide sequence SWTLYTPSGQSK that can specifically bind to Ncadherin.The peptide BFS can form nanoparticles in PBS,which can transform to nanofibers when targeting and binding to N-cadherin.The nanofibers inhibit the migration of N-cadherin overexpressed MDA-MB-436 cancer cells.The peptide BFS shows 83.6%inhibiting rate in cells wound healing assay.In addition,the inhibition rate is 67.9%when the BFS applied in transwell migration assay.These results indicate that the BFS has excellent ability to inhibit migration of cancer cells.This self-assembly strategy could be potentially utilized to regulate the key protein during EMT for inhibiting the tumor metastasis.

关 键 词：SELF-ASSEMBLY PEPTIDE N-CADHERIN Cancer Ligand-receptor interaction 

分 类 号：TB383.1[一般工业技术—材料科学与工程] R730.5[医药卫生—肿瘤]