Cholesterol as a functional metabolite cooperates with metadherin in cancer cells  

作　　者：Wen Wang Xiaolong Liu Huan Chen Ting Ling Tian Xia Xiumei Liu Jing Liu Wuxiyar Otkur Xianzhe Shi Huan Qi Di Chen Hai-Long Piao 

机构地区：[1]CAS Key Laboratory of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Chinese Chemical Letters》2020年第7期1831-1834,共4页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(Nos.81672440,21575142);Innovation Program of Science and Research from the DICP,CAS(No.DICP ZZBS201803)。

摘　　要：Protein-metabolite interactions(PMIs)play important roles in various biological processes,especially in disease progression.However,due to the complexity of living cells,it is very difficult to identify specific PMIs.Herein,we chose one oncogenic factor,metadherin(MTDH),as a bait to identify its in vivo interacting metabolites in cancer cells.Cholesterol is an important metabolite and essential structural component of cell membranes.It could also drive several diseases including cancer.Interestingly,we found that cholesterol robustly interacted with MTDH and downregulated the expression of MTDH in cancer cells.Furthermore,MTDH disturbed metabolite alterations under cholesterol treatment in MTDH transduced cancer cells.Collectively,our results uncover an undescribed PMI where MTDH,as an oncogenic factor,might positively regulate cancer progression by interacting with choleste rol.This study interprets the theoretical basis of PMI-oriented cancer progression and targeting therapies in clinic.

关 键 词：Metabolite-protein interaction CHOLESTEROL Metadherin Mass spectrometry 

分 类 号：R730.5[医药卫生—肿瘤]