Establishment of cell lines with porcine spermatogonial stem cell properties  

作　　者：Yi Zheng Tongying Feng Pengfei Zhang Peipei Lei Fuyuan Li Wenxian Zeng 

机构地区：[1]Key Laboratory for Animal Genetics,Breeding and Reproduction of Shaanxi Province,College of Animal Science and Technology,Northwest A&F University,Yangling 712100,Shaanxi,China

出　　处：《Journal of Animal Science and Biotechnology》2020年第3期678-689,共12页畜牧与生物技术杂志（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(Grant No.31572401,31772605)to W.Z.;the Open Fund of Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province(Grant No.SNDK-KF-201804);Young Talent fund of University Association for Science and Technology in Shaanxi,China(Grant No.20180204)and a startup fund from Northwest A&F University(Grant No.2452018037)to Y.Z.

摘　　要：Background:Spermatogonial stem cells(SSCs)are capable of both self-renewal and differentiation to mature functional spermatozoa,being the only adult stem cells in the males that can transmit genetic information to the next generation.Porcine SSCs hold great value in transgenic pig production and in establishment of porcine models for regenerative medicine.However,studies and applications of porcine SSCs have been greatly hampered by the low number of SSCs in the testis as well as the lack of an ideal stable long-term culture system to propagate porcine SSCs perpetually.Results:In the present study,by lentiviral transduction of plasmids expressing the simian virus 40(SV40)large T antigen into porcine primary SSCs,we developed two immortalized cell lines with porcine SSC attributes.The established cell lines,with the expression of porcine SSC and germ cell markers UCHL1,PLZF,THY1,VASA and DAZL,could respond to retinoic acid(RA),and could colonize the recipient mouse testis without tumor formation after transplantation.The cell lines displayed infinite proliferation potential,and have now been cultured for more than 7 months and passaged for over 35 times without morphological abnormalities.Conclusions:We have for the first time established porcine SSC lines that could provide abundant cell sources for mechanistic studies on porcine SSC self-renewal and differentiation,thereby facilitating development of an optimal long-term culture system for porcine primary SSCs and their application to animal husbandry and medicine.

关 键 词：IMMORTALIZATION PIG SELF-RENEWAL Spermatogonial stem cells SV40 large T antigen 

分 类 号：S828.2[农业科学—畜牧学]